30,258 results

1. pJC175e

   Plasmid

   #79219

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PPSP gIII, luxAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PPSP

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSETb-pcDronpa2

   Plasmid

   #78184

   Purpose

   Expresses pcDronpa2 in E. coli strains

   Depositor

   Peter Dedecker

   Article

   Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

   Insert

   pcDronpa2
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHN781

   Plasmid

   #178828

   Purpose

   Bacterial expression for human Saposin A

   Depositor

   Kazuhiro Abe

   Insert

   artificial gene encoding human Saposin A (AR Human)
   

   Use

   Tags

   His8, TEV

   Expression

   Bacterial

   Mutation

   Codon-usage was optimized for bacterial expressio…

   Promoter

   T7

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GST-RH▲-MNase

   Plasmid

   #136292

   Purpose

   Expression of catalytic inactive RNase H fused to micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   RNase HI-D10R-E48R and MNase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SPLICSFAST-PO-MT-P2A

   Plasmid

   #214899

   Purpose

   Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-PO-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7II-2N4Rtau

   Plasmid

   #177653

   Purpose

   Recombinant protein synthesis of human Tau

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   Tau (2N4R) (MAPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-Sac-Abeta(M1-40)

   Plasmid

   #71876

   Purpose

   Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-40) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. R701-X65-527: His6-tev-Hs.CALM1(2-148)

   Plasmid

   #159693

   Purpose

   E. coli protein expression of His6-tev-Hs.CALM1(2-148)

   Depositor

   Dominic Esposito

   Article

   Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

   Insert

   His6-tev-Hs.CALM1(2-148) (CALM1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. keratin 8 (krt8)-H2B-RFP

   Plasmid

   #105955

   Purpose

   transgenesis

   Depositor

   Caren Norden

   Article

   Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

   Insert

   krt8 (KRT8 Human)
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SPLICSFAST-ER-MT-P2A

    Plasmid

    #214892

    Purpose

    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GST-MNase

    Plasmid

    #136291

    Purpose

    Expresses Micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-gp32-H6

    Plasmid

    #163912

    Purpose

    Expresses T4 gp32 for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    gp32
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD24-sfGFPx1

    Plasmid

    #51558

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SPLICSFAST-NU-MT-P2A

    Plasmid

    #214898

    Purpose

    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-NU-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pProEx RalA(G23C) CL

    Plasmid

    #224263

    Purpose

    Protein purification of RalA (G23C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    RalA (RALA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G23C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXalb_IC_Cascade

    Plasmid

    #178765

    Purpose

    Expression of X. albilineans type I-C Cascade genes

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    X. albilineans type I-C Cascade genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-SrtAdelta59

    Plasmid

    #51138

    Purpose

    expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.aureus SrtA delta 59
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pREDawn-AmpR-MCS

    Plasmid

    #188978

    Purpose

    For red light-induced bacterial expression of gene of interest (AmpR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT-HA-rM3D(Gs)

    Plasmid

    #45549

    Purpose

    Expresses rM3D (Gs) neuronal activator

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    rM3D (Gs)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge