30,736 results
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Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterP(rhaB)Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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pLinker-BirA-3xHA-HXGPRT-loxP
Plasmid#86668Purposefor generation of BirA tagged strains using CRISPR tagging technologyDepositorInsertLinker-BirA-3xHA-HXGPRT 3'UTR
ExpressionBacterialAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBDC
Plasmid#135188PurposeIntegration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.DepositorInsertCmR
UseSynthetic BiologyExpressionBacterialMutationNonePromotercatAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
TagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-KRaION1
Plasmid#177799PurposeBacterial expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
ExpressionBacterialPromoteraraBAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag
Plasmid#109030PurposeWild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53-DsbC
Plasmid#186623PurposeE. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.DepositorTypeEmpty backboneTagsDsbC-6His-TEVExpressionBacterialPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
LARS_pNIC-Bio3
Plasmid#153052PurposeExpression construct for producing Avi tagged Leucyl-tRNA synthetaseDepositorInsertLARS (LARS1 Human)
TagsAvi and His6-TEVExpressionBacterialMutationNot full length proteinPromoterT7Available SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet-I
Plasmid#104007PurposePlasmid encoding mScarlet-IDepositorInsertmScarlet-I
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUX19
Plasmid#36328DepositorTypeEmpty backboneUseSuicide vector for r. rubrumExpressionBacterialPromoterreduce strength lacZAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
TARS_pNIC-Bio3
Plasmid#153057PurposeExpression construct for producing Avi tagged Threonyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL003
Plasmid#215335PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)DepositorTypeEmpty backboneExpressionBacterialPromoterpSpankAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCF158 Human beta-2 microglobulin
Plasmid#180457PurposeE. coli expression of class I MHC light chainDepositorInsertHuman beta-2 microglobulin
ExpressionBacterialAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET29b-Rhobin2
Plasmid#239018PurposeBacterial overexpression of de novo Rhodamine binder 2 (Rhobin2) proteinDepositorInsertRhoBin2
Tags6xHisExpressionBacterialPromoterT7Available SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PunyEEER
Plasmid#208322PurposeBacterial expression of the His-tagged peptide PunyEEER.DepositorInsertPunyERRE
TagsDBCO-Tag, His-TagExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-TinyERRE
Plasmid#208320PurposeBacterial expression of the His-tagged peptide TinyERRE.DepositorInsertTinyERRE
TagsDBCO-Tag, His-TagExpressionBacterialAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 2A-CVB3
Plasmid#202076PurposeBacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 2A protease with HisTag
Tags6xHis-tagExpressionBacterial, Insect, and Mamm…Promoterpolh, CAG, T7Available SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDx_mCherry-SYFP2
Plasmid#189759PurposeDual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysisDepositorInsertmCherry-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only