34,359 results

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pTrcHIS-wt7D12

Plasmid

#125268

Purpose

Expresses wild type 7D12 anti-EGFR nanobodies

Depositor

Angus Johnston

Publication

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 nanobody

Tags

His-Tag

Expression

Bacterial

Promoter

Trc

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-Bxb1-His

Plasmid

#123132

Purpose

Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

Depositor

David Liu

Publication

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Bxb1 integrase

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET29b-AK1-His

Plasmid

#124135

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Adenylate kinase (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-21a(+)-AK1-His

Plasmid

#118977

Purpose

Bacterial expression plasmid for Adenylate kinase isoenzyme 1

Depositor

Sebastian Maerkl

Publication

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PB-TRE3G-SOX17

Plasmid

#104541

Purpose

PiggyBac vector encoding doxycycline-inducible human SOX17

Depositor

David Vereide

Publication

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

SRY-box 17 (SOX17 Human)

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD-LSSmCherry1

Plasmid

#89986

Purpose

Long stokes-shift variant of red fluorescent protein mCherry

Depositor

Robert Campbell

Publication

Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

Insert

LSSmCherry1

Tags

6xHis-T7-Xpress

Expression

Bacterial

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLPT107

Plasmid

#85525

Purpose

Repressilator with triple reporter

Depositor

Johan Paulsson

Publication

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI
mKate2

Use

Synthetic Biology

Tags

ssrA

Expression

Bacterial

Available since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Publication

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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NowGFP /pQE-30

Plasmid

#74749

Purpose

NowGFP gene for bacterial expression

Depositor

Konstantin Lukyanov

Publication

Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

Insert

NowGFP

Tags

HisTag (with a mutation but still working)

Expression

Bacterial

Mutation

WasCFP with substitutions E6K, L42M, T43S, V68M, …

Promoter

T5

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEX4T-hLIG3

Plasmid

#81055

Purpose

bacterial expression of human LIG3

Depositor

Primo Schaer

Publication

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

DNA ligase 3 (LIG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET31b-T7-Spinach2

Plasmid

#79783

Purpose

Expresses Spinach2 under a T7 promoter

Depositor

Ming Hammond

Publication

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2

Expression

Bacterial

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUCP20T-eyfp

Plasmid

#78466

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Publication

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eYFP

Expression

Bacterial

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTKW106alp7A

Plasmid

#69360

Purpose

Stabilized lacZ expression vector containing alp7A and hok/sok

Depositor

Jeff Hasty

Publication

Danino et al Sci Transl Med. 2015 May 27;7(289):289ra

Inserts

lacZ
alp7A
hok
placIq-lacI

Expression

Bacterial

Promoter

pTac and placIq

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET15b SARS-CoV PLpro

Plasmid

#61693

Purpose

inducible expression of SARS PLpro protease

Depositor

Michael Johnson

Publication

Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

Insert

SARS-CoV PLpro

Tags

His

Expression

Bacterial

Promoter

T7

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-His-hAos1

Plasmid

#53135

Purpose

E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

Depositor

Frauke Melchior

Publication

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Aos1 (SAE1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mOrange-pBAD

Plasmid

#54751

Purpose

Localization: Protein Expression Vector , Excitation: 548, Emission: 562

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and mOrange

Expression

Bacterial

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pST-K

Plasmid

#44560

Purpose

for ectopic constitutive expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Publication

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Promoter

UV15 promoter

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDMS197

Plasmid

#43831

Depositor

Dieter Schifferli

Publication

Edwards et al Gene. 1998 Jan 30;207(2):149-57.

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb1

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

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PBAD-his6-prkA-pACYC184

Plasmid

#41041

Depositor

Ichiro Matsumura

Publication

Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

Insert

prkA

Tags

6xHis and T7 epitope tag

Expression

Bacterial

Promoter

PBAD

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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GST-PRMT6

Plasmid

#34700

Depositor

Steven Clarke

Publication

Frankel et al J Biol Chem. 2002 Feb 1;277(5):3537-43.

Insert

PMRT6

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only