30,135 results

1. L4440_BioBrick-hsf-1-sgRNA-B

   Plasmid

   #177787

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hsf-1 (hsf-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBb(RSF1010)1k-GFPuv

   Plasmid

   #106395

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGDP3 rmtB

   Plasmid

   #112880

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   rmtB (RmtB Escherichia coli)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGDP4 ermC

   Plasmid

   #112884

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   ermC (ermM Staphylococcus epidermis)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOLA-Gent-EM7-Erv1p-PDI

   Plasmid

   #202486

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   EM7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGDP3 arr

   Plasmid

   #112890

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   arr
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a- mEGFP-kanMX6

   Plasmid

   #105146

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu, Thomas Pollard

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   mEGFP-kanMX6
   

   Use

   Tags

   mEGFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ2072.2_c1

   Plasmid

   #140675

   Purpose

   CRISPRlator control (“open ring”)

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   CRISPRlator control (“open ring”)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGDP3 cat

    Plasmid

    #112888

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    cat (Brevibacillus brevis) (cat Brevibacillus brevis)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGDP1 fosA

    Plasmid

    #112889

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    fosA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRAU168

    Plasmid

    #86834

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA1

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGDP1 vph

    Plasmid

    #112893

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    vph
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGDP3 vatD

    Plasmid

    #112885

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    vatD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGDP3 aph(3'')-Ia

    Plasmid

    #112879

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGDP3 aph(9)-Ia

    Plasmid

    #112882

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    aph(9)-Ia
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psfTn5

    Plasmid

    #79107

    Purpose

    bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

    Depositor

    Sten Linnarsson

    Insert

    transposase 5
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E54K and L372P

    Promoter

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. prhaBAD-anti-mouse-Nb-Hia5

    Plasmid

    #239626

    Purpose

    Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

    Insert

    Hia5
    

    Use

    Tags

    6HIS, MBP, TEV, and anti-Mouse Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. prhaBAD-anti-rabbit-Nb-Hia5

    Plasmid

    #239627

    Purpose

    Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Sidhwani et al bioRxiv 2025.07.01.662447; doi: https://

    Insert

    Hia5
    

    Use

    Tags

    6HIS, MBP, TEV, and anti-Rabbit Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG GST Empty

    Plasmid

    #236735

    Purpose

    Empty bacterial expression plasmid for GST fusion proteins

    Depositor

    Melina Fan, Addgene Research Program

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge