33,902 results
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pGEX-NEMO
Plasmid#11965DepositorPublicationAvailable sinceJuly 13, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIVM_26
Plasmid#204501PurposeExpression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)DepositorExpressionBacterialMutation0PromoterAmpRAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
TagsHis-tag followed by thrombin cleavage siteExpressionBacterialPromoterT7Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
TagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
ExpressionBacterial and YeastAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETduet+p300HAT
Plasmid#157793PurposeExpression of p300 HAT domainDepositorAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046828
Plasmid#145742PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
TagsMPBExpressionBacterialPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc
Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseExpressionBacterialAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
Tags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPEPY-PF6-lacI
Plasmid#85589PurposeWork as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniaeDepositorInsertlacI
ExpressionBacterialPromoterPF6Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-PuroDTK.Neo
Plasmid#72307PurposePiggyBac Cassette contains puromycin and TK genesDepositorInsertPuromycin-DTK-neo
ExpressionBacterial and MammalianPromoterCAG promoterAvailable sinceFeb. 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M13KO7-ΔM13-ΔgeneIII-ΔgeneVI
Plasmid#80840PurposeModified helper phageDepositorInsertsM13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV
ExpressionBacterialAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUltra-sY
Plasmid#82417PurposeExpresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codonsDepositorInsertMethanococcus jannaschii aaRS for sulfotyrosine
ExpressionBacterialMutationTyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…PromotertacIAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SRC_HUMAN_D0
Plasmid#79700PurposeThis plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
TagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINS-UBE3C
Plasmid#66711PurposeExpresses UBE3C HECT domain in E. coliDepositorAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-Tight-Puro_Sox10
Plasmid#46536PurposeA lentiviral vector for the expression of mouse Sox10 under doxycyline control.DepositorAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by