30,736 results
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pET11b-MIF
Plasmid#149446PurposeExpression of WT MIF in E. coli for protein productionDepositorAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST44
Plasmid#64007Purposepolycistronic cloning vector for pST44 plasmid suiteDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSNA
Plasmid#135192PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyExpressionBacterialMutationNonePromoteraraBADAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBad-PhoCl2f
Plasmid#164036PurposeImproved PhoCl variant with faster dissociation rateDepositorInsertPhoCl2f
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_15bpLinker
Plasmid#157786PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_15bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPP381
Plasmid#158798PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purificationDepositorInsertdCasPhi-2
Tags6xHisExpressionBacterialMutationRuvC-inactivating mutation D394AAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15bpATnp
Plasmid#121137PurposeThis vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.DepositorInsertProtein A-Tnp
TagshisExpressionBacterialPromoterT7Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIV151
Plasmid#226180PurposeExpresses Cas9 in a backbone compatible with K. phaffii.DepositorInsertCas9
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterGAP1Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCJ217
Plasmid#162685PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
TagsHisExpressionBacterialMutationG489C, S530C; codon optimized for E. coli K12PromoterT7/lacAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-pBAD
Plasmid#54723PurposeLocalization: Protein Expression Vector , Excitation: 516, Emission: 529DepositorTypeEmpty backboneTags6xHis and mCitrineExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
ABE8e-Cas9NG
Plasmid#203328PurposePlasmid for bacterial purification of codon optimized ABE8e-Cas9NGDepositorInsertABE8e-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHJL4
Plasmid#199379PurposeEncodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP7f::SL2::mScarlet-I
ExpressionBacterialMutationno mutationsAvailable SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNeae2
Plasmid#168300PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
Tags6xHis, E-tag, and myc-tagExpressionBacterialPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSI-1
Plasmid#51725Purposecloning backboneDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBZS-M.CviQIX (NDel15)
Plasmid#198357PurposeExpress M.CviQIX (NDel15) in bacterial cells.DepositorInsertM.CviQIX
ExpressionBacterialAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130A wgMDH
Plasmid#204266PurposeBacterial expression of R130A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR130AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-CD63
Plasmid#194989Purposeoverexpress CD63DepositorInsertGFP-CD63
ExpressionBacterialAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-165
Plasmid#118577PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
