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pET22b-CPDSalI

Plasmid

#38251

Depositor

Matthew Bogyo, Aimee Shen

Publication

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)

Plasmid

#29723

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mO…

Expression

Bacterial

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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monomer-HD

Plasmid

#27968

Depositor

Feng Zhang

Publication

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-monomer-HD

Expression

Bacterial

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28GST-LIC

Plasmid

#26101

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6XHis, GST, and Thrombin CLeavage Site

Expression

Bacterial

Available since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pACYC-FLAG-dN6-His

Plasmid

#22143

Depositor

Robert Sauer

Publication

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

Flag-clpXdeltaNLinkedHexamer-His6

Tags

Flag, His6, and L20 Linkers

Expression

Bacterial

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTC-28S15-0X

Plasmid

#20316

Depositor

Dirk Schnappinger

Publication

Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

Insert

Psmyc-tetR#28

Expression

Bacterial

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET3a-Yuh1

Plasmid

#18895

Depositor

Cecile Pickart

Publication

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)

Expression

Bacterial

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pB1H2w2-mutOdd

Plasmid

#18044

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

mutant Odd Skipped

Expression

Bacterial

Mutation

A mutant version of the zinc finger protein Odd S…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pENTR FOXP3

Plasmid

#16363

Depositor

Anjana Rao

Publication

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Tags

Myc

Expression

Bacterial

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Pdx1-RFP puro (DM#235)

Plasmid

#15085

Depositor

Douglas Melton

Publication

Rajagopal et al Science. 2003 Jan 17. 299(5605):363.

Insert

Pdx1 promoter (Pdx1 Mouse)

Tags

RFP

Expression

Bacterial

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
Dlx5

Plasmid

#15538

Depositor

John Rubenstein

Publication

Zerucha et al J Neurosci. 2000 Jan 15. 20(2):709-21.

Insert

Dlx5 (Dlx5 Mouse)

Expression

Bacterial

Available since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX-NEMO

Plasmid

#11965

Depositor

Jon Ashwell

Publication

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Tags

GST

Expression

Bacterial

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Publication

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Expression

Bacterial

Available since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pIVM_02

Plasmid

#204500

Purpose

Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

Depositor

Alessio Ciulli

Publication

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

amino acids 54-213 VHL (VHL Human)

Tags

6xHis, TEV, and Thrombin

Expression

Bacterial

Mutation

0

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep

Plasmid

#173899

Purpose

The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr

Depositor

Jason W Chin

Publication

Zhang et al Nat Methods. 2017 Jul;14(7):729-736. doi

Inserts

ThrRS(v1.0)
tRNA(v2.0)CUA
pduX
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-Tev

Plasmid

#177142

Purpose

Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

Depositor

Robert Sobol

Publication

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

Tev

Tags

GST

Expression

Bacterial

Promoter

Tac

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-GST-His6-2_HBD

Plasmid

#171870

Purpose

Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

Depositor

Kaiwei Liang

Publication

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Tandem repeats Hybrid binding domain of Rnase H1

Tags

GST- and His10-tag

Expression

Bacterial

Mutation

WT

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-sgRNA-RFP

Plasmid

#166005

Purpose

Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

Depositor

Martin Buck

Publication

Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Publication

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046828

Plasmid

#145742

Purpose

Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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