30,416 results

1. pACYCDuet1-tglB

   Plasmid

   #134676

   Purpose

   Expresses His6-TglBin E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Insert

   TglB
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-pBAD

   Plasmid

   #54762

   Purpose

   Localization: Protein Expression Vector , Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Use

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBR322-TIMER

   Plasmid

   #103056

   Purpose

   medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE 80L Kan T7WT

   Plasmid

   #102790

   Purpose

   Expresses T7 RNA polymerase

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   T7 RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 3, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pE-FLP

   Plasmid

   #45978

   Purpose

   Expresses yeast Flp driven by the constitutive promoter pE from phage P2

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Insert

   flp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   flp driven by the constitutive promoter pE from p…

   Promoter

   pE (from phage P2)

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMA7CR_2.0

   Plasmid

   #73950

   Purpose

   pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Inserts

   cas9
   lambda beta
   dam
   recX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pAra and pTet

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-RvLEAMshort

   Plasmid

   #218122

   Purpose

   Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

   Depositor

   Ci Ji Lim

   Article

   Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

   Insert

   RvLEAMshort
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Truncated form (58-181aa)

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM1s3AsG

   Plasmid

   #137921

   Purpose

   E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   Superfolder GFP
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pM2s2TsR

    Plasmid

    #137923

    Purpose

    E. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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