30,416 results

1. pET30-2-GAPDH

   Plasmid

   #83910

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pE-SUMO CRYGD

   Plasmid

   #80755

   Purpose

   Expression of human gamma D-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYGD (CRYGD Human)
   

   Use

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL1765 (pSPIN-R, R*MmeI)

   Plasmid

   #160734

   Purpose

   Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-sfGFP

   Plasmid

   #85492

   Purpose

   pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b-AavLEA1

   Plasmid

   #53093

   Purpose

   AavLEA1 expression (in BL21 DE3 E. coli strain)

   Depositor

   Claude Férec

   Article

   Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

   Insert

   AavLEA1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET16B.Pfu

   Plasmid

   #12509

   Purpose

   Bacterial expression of Pfu DNA polymerase

   Depositor

   Sung-Hou Kim

   Article

   Goldman et al Acta Crystallogr D Biol Crystallogr. 199

   Insert

   Pfu DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y218H, Q462R, F533L, K559I and V683A (mutations w…

   Promoter

   Available Since

   Sept. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH6HTC_PRDX6-Halo

   Plasmid

   #175338

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PRDX6 (PRDX6 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAZ-SK

   Plasmid

   #73962

   Purpose

   self-killing gRNA plasmid pMAZ-SK

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpyTag003-MBP

   Plasmid

   #133450

   Purpose

   Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT FL USP7

    Plasmid

    #227214

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7-promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge