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pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-SF2

Plasmid

#99020

Purpose

Bacterial expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Tags

GST

Expression

Bacterial

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMB1-A-TelR

Plasmid

#204979

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16

Plasmid

#153524

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#16

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#16

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Tags

6xHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T HHARI RBR

Plasmid

#31254

Depositor

Rachel Klevit

Article

Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

Insert

Human Homologue of Ariadne (HHARI) (ARIH1 Human)

Tags

GST

Expression

Bacterial

Mutation

RBR domain residues 177-395

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pBAD-luxKate

Plasmid

#229946

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKate

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFCiR5

Plasmid

#149479

Purpose

Broad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET24a-AlkB deltaN11

Plasmid

#73622

Purpose

Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

Depositor

Catherine Drennan

Article

Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

AlkB (alkB E. coli)

Tags

His

Expression

Bacterial

Mutation

deletion of first 11 AA

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pOPINN-GFP

Plasmid

#53541

Purpose

pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

Depositor

Ray Owens

Insert

GFP

Tags

N-His-GFP

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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