30,416 results

1. HSA3_His

   Plasmid

   #202765

   Purpose

   human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA3
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDB179-HotPETase

   Plasmid

   #218701

   Purpose

   Encodes codon optimized HotPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   HotPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. psfTn5

   Plasmid

   #79107

   Purpose

   bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

   Depositor

   Sten Linnarsson

   Insert

   transposase 5
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E54K and L372P

   Promoter

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCT5-bac1.8

   Plasmid

   #119871

   Purpose

   Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

   Depositor

   Claudia Schmidt-dannert

   Article

   Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

   Inserts

   CymR'
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   SNP in 592 bp

   Promoter

   PserA promoter and Pveg promoter with CuO sequence

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET51b-His-TEV-HaloTag7

   Plasmid

   #167266

   Purpose

   Overexpression of HaloTag7 in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HaloTag7
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC18T-mini-Tn7T-Gm-dsRedExpress

   Plasmid

   #65032

   Purpose

   GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   dsRedExpress
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 14, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBad-sfGFP 150TAG

   Plasmid

   #85483

   Purpose

   Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   superfolder GFP 150TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   150TAG (amino acid 150 mutated to stop codon)

   Promoter

   Arabinose

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30a-AlkB-D135S

   Plasmid

   #79051

   Purpose

   Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-GST

   Plasmid

   #42049

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   GST
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGRG36

    Plasmid

    #16666

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

    Insert

    mTn7::MCS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge