29,996 results

1. T444T

   Plasmid

   #113081

   Purpose

   Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH-MBP-CbAgo

   Plasmid

   #127707

   Purpose

   pET His6 MBP TEV CbAgo

   Depositor

   John van der Oost

   Article

   Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

   Insert

   CbAgo
   

   Use

   Tags

   MPB

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEVI(408)-ColE-iLight-msfGFP

   Plasmid

   #170268

   Purpose

   Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   iLight
   

   Use

   Tags

   msfGFP

   Expression

   Bacterial

   Mutation

   To design iLight for expression in bacteria, a fu…

   Promoter

   pJ23116

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBHM2403

   Plasmid

   #171687

   Purpose

   pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   CnoCAS9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTB3-BC2 nanobody

   Plasmid

   #210208

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   BC2 Nanobody
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Sac-Abeta(M1-40)

   Plasmid

   #71876

   Purpose

   Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-40) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-SMAD4

   Plasmid

   #16491

   Depositor

   Bert Vogelstein

   Article

   Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

   Insert

   Smad4 (SMAD4 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

8. BirA pLEMO

   Plasmid

   #119818

   Purpose

   Express endogenous biotin ligase in E.coli

   Depositor

   Alexander Gabibov

   Article

   Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

   Insert

   Biotin ligase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Rhamnose promoter

   Available since

   April 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. KARS_pNIC-Bio3

   Plasmid

   #153051

   Purpose

   Expression construct for producing Avi tagged Lysyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   KARS (KARS1 Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pREDusk-MCS

    Plasmid

    #188970

    Purpose

    For red light-repressed bacterial expression of gene of interest

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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