Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,786 results

Showing: 1621 - 1640 of 31786 results

  4. pFC902

    Plasmid
    #106904
    Purpose
    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108
    Inserts
    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106904-plasmid-map-sequence-id-206325

    Enlarge

  9. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

    Enlarge

  11. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid
    #218191
    Purpose
    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Article
    Rieubon et al protocols.io 2025
    Insert
    nucA
    Tags
    NSP4 signal secretion peptide and myc
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218191-plasmid-map-sequence-id-433865

    Enlarge

  13. pDNR-3xFLAG-T2A-3xPuroR-Frame 0

    Plasmid
    #245866
    Purpose
    HITAG Donor plasmid for Frame 0 with 3xFLAG tag and triple Puromycin resistant marker (3xPuroR)
    Depositor
    Alejandro Chavez
    Insert
    puromycin resistant marker
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    Endogenous gene promoter after knock-in
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245866-plasmid-map-sequence-id-491961

    Enlarge

  14. pDNR-3xFLAG-T2A-3xPuroR-Frame 1

    Plasmid
    #245867
    Purpose
    HITAG Donor plasmid for Frame 1 with 3xFLAG tag and triple Puromycin resistant marker (3xPuroR)
    Depositor
    Alejandro Chavez
    Insert
    ampicilin resistant marker
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245867-plasmid-map-sequence-id-491963

    Enlarge

  15. pDNR-3xFLAG-T2A-3xPuroR-Frame 2

    Plasmid
    #245868
    Purpose
    HITAG Donor plasmid for Frame 2 with 3xFLAG tag and triple Puromycin resistant marker (3xPuroR)
    Depositor
    Alejandro Chavez
    Insert
    3xFLAG
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245868-plasmid-map-sequence-id-491964

    Enlarge

  17. pFN1-Aad9

    Plasmid
    #244449
    Purpose
    A shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a spectinomycin resistance marker (aadA gene).
    Depositor
    Xue Liu
    Inserts
    pFn1 Fn ori
    Aad9
    Expression
    Bacterial
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    244449-plasmid-map-sequence-id-490024

    Enlarge

  18. pBI121::smRatioGFP:AQ

    Plasmid
    #240453
    Purpose
    Expression of indicator protein fusion (soluble modified ratiometric pHluorin & Aequorin) for monitoring calcium concentrations and pH in the cytoplasm of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of soluble modified ratiometric pHluorin and Aequorin
    Expression
    Bacterial and Plant
    Mutation
    Ratiometric GFP (ratiometric pHluorin) (PMID 9671…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240453-plasmid-map-sequence-id-485325

    Enlarge

  19. pBI121::chitEclipGFP:LAAQ

    Plasmid
    #240452
    Purpose
    Expression of indicator protein fusion (modified ecliptic pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of Chitinase signal, soluble modified ecliptic pHluorin and low affinity Aequorin (D119A)
    Expression
    Bacterial and Plant
    Mutation
    Ecliptic GFP (ecliptic pHluorin) (PMID 9671304); …
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240452-plasmid-map-sequence-id-485324

    Enlarge

Showing: 1621 - 1640 of 31786 results