30,535 results

1. esmGFP-C10-pVTCB052

   Plasmid

   #231303

   Purpose

   Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli

   Depositor

   Alexander Rives

   Insert

   esmGFP-C10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b-His-TEV-SNAP-tag

   Plasmid

   #167269

   Purpose

   Overexpression of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDB302

   Plasmid

   #113673

   Purpose

   expresses Cth SUMO protease in Escherichia coli

   Depositor

   Christopher Bahl

   Article

   Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

   Insert

   Cth SUMO protease
   

   Use

   Tags

   10-His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-934

   Promoter

   T7

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ATX3-13Q

   Plasmid

   #184248

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
   

   Use

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   C-terminal codon optimization for protein expres…

   Promoter

   T7 promoter

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiex-1 AC30_eGFP

   Plasmid

   #82715

   Purpose

   Expresses a GFP-tagged recombinant adenylyl cyclase.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
   

   Use

   Tags

   FLAG purification tag and eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pInt

   Plasmid

   #18941

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   under lacZ promoter

   Promoter

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH6HTC_IGF2BP2a-Halo

   Plasmid

   #175309

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   IGF2BP2 (IGF2BP2 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-TetOn-dCas9-KRAB

   Plasmid

   #115545

   Purpose

   Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDae079 (eMutaT7[transition])

   Plasmid

   #187622

   Purpose

   Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

   Inserts

   TadA-8e-T7RNAP
   PmCDA1-T7RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSL1-GFP

    Plasmid

    #180420

    Purpose

    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

    Depositor

    Nancy Moran

    Article

    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-SUMO-Rad23A_pET11a

    Plasmid

    #169124

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hRad23A (RAD23A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. BRD4

    Plasmid

    #38942

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMB67EH-YFP

    Plasmid

    #90104

    Purpose

    YFP control expression plasmid

    Depositor

    Samuel Miller

    Article

    Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

    Insert

    YFP
    

    Use

    Broad-host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCold CL7-Cas9

    Plasmid

    #124890

    Purpose

    obtaining full Cas9 RNP

    Depositor

    Yi Liu

    Article

    Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJ404-DogCatcher-sfGFP

    Plasmid

    #171930

    Purpose

    Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    DogCatcher-sfGFP
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAJM.661

    Plasmid

    #108532

    Purpose

    TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    TtgRAM + PTtg-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TtgR: R130H A144T A148V

    Promoter

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSF-KpAGO207-1251

    Plasmid

    #117854

    Purpose

    Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

    Depositor

    Dinshaw Patel, Kotaro Nakanishi

    Article

    Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

    Insert

    Argonaute
    

    Use

    Tags

    6xHis-SUMO encoded in vector

    Expression

    Bacterial

    Mutation

    missing first 206 amino acid residues

    Promoter

    T7

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. GSK3 beta pGEX

    Plasmid

    #15898

    Depositor

    Jim Woodgett

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 1, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. human pirh2-(1-261)

    Plasmid

    #24878

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only