30,738 results

1. pB-Rex

   Plasmid

   #122134

   Purpose

   B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

   Depositor

   Srivatsan Raman

   Article

   Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

   Insert

   B. subtilis Rex
   

   Expression

   Bacterial

   Promoter

   apFAB338

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDB6 (CK2alpha')

   Plasmid

   #27084

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha' (CSNK2A2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDIV270

   Plasmid

   #226181

   Purpose

   Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   TEF1p (Komagataella phaffii)

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETNb-cALFA

   Plasmid

   #159989

   Purpose

   For the protein expression with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDB179-TurboPETase

   Plasmid

   #218693

   Purpose

   Encodes codon optimized TurboPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   TurboPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAB167

   Plasmid

   #101672

   Purpose

   Opto-T7RNAP*(563), equal expr.

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(563), equal expr.
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRF-TetR

   Plasmid

   #49374

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   TetR repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET GFP LIC cloning vector (u-msfGFP)

   Plasmid

   #29772

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   SiriusGFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVoPo-mCh

   Plasmid

   #187841

   Purpose

   Constitutive mCherry-expression vector for Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKCyR8

    Plasmid

    #149494

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CymR(AM)-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PCymRC

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Human Parkin (1-465)

    Plasmid

    #110758

    Purpose

    Bacterial expression of human Parkin

    Depositor

    David Komander

    Article

    Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

    Insert

    PRKN (PRKN Human)
    

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRC-APEX2

    Plasmid

    #72558

    Purpose

    Expression of cytosolic APEX2 in bacteria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A134P relative to APEX

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTriExRhoA2G

    Plasmid

    #40176

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET MBP mCherry LIC cloning vector (MBP-mCherry)

    Plasmid

    #29747

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-MinE-His

    Plasmid

    #133623

    Purpose

    Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

    Insert

    MinE-His (minE E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. phage UbiC tagRFP-T-DDX6

    Plasmid

    #119947

    Purpose

    Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    DDX6 (DDX6 Human)
    

    Use

    Lentiviral

    Tags

    tagRFP-T

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF1-Pls1-sfGFP

    Plasmid

    #205748

    Purpose

    Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.

    Depositor

    Jerome Bonnet

    Article

    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

    Type

    Empty backbone

    Use

    Shuttle vector gram+ gram-

    Expression

    Bacterial

    Promoter

    Pls1 (Phyperspank mutant, IPTG inducible)

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET23a-H6-TEV-cSrc

    Plasmid

    #214233

    Purpose

    Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    cSrc Kinase domain (SRC Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET24a-VHH-tev-mCherry

    Plasmid

    #117752

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge