29,691 results

1. pJS169

   Plasmid

   #48882

   Purpose

   Negative feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Insert

   lacI with ssrA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   Para/lac

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7C-HSPA8

   Plasmid

   #177661

   Purpose

   Recombinant protein synthesis of human HSPA8

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSPA8 (HSPA8 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pλPR cI-Elysis

   Plasmid

   #176891

   Purpose

   Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

   Depositor

   James Inglese

   Article

   Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

   Insert

   Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJYS3_ΔcrtYf

   Plasmid

   #85542

   Purpose

   Constitutive transcription of FnCpf1 and crRNA of crtYf

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21c(+)-mtLeuRS

   Plasmid

   #31079

   Depositor

   Linda Spremulli

   Article

   Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

   Insert

   Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pMRE-Tn5-132

   Plasmid

   #118518

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pet22b-mEGFP

   Plasmid

   #166439

   Purpose

   Bacterial expression of 6x His tagged mEGFP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGTM_YR375_SUMO_Protease_001

   Plasmid

   #190063

   Purpose

   Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   ULP1_YEAST (ULP1 Budding Yeast)
   

   Use

   Tags

   8X His-MBP (Maltose Binding Protein)

   Expression

   Bacterial

   Mutation

   Construct contains residues 347 – 621

   Promoter

   T7 lac

   Available since

   Jan. 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBHRSF184

   Plasmid

   #89482

   Purpose

   Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

   Depositor

   Hideo Iwai

   Article

   Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

   Insert

   Asparaginyl endopeptidase from Oldenlandia affinis
   

   Use

   Tags

   Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACYC-FLAG-dN6-His

    Plasmid

    #22143

    Depositor

    Robert Sauer

    Article

    Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

    Insert

    Flag-clpXdeltaNLinkedHexamer-His6
    

    Use

    Tags

    Flag, His6, and L20 Linkers

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZac2.1 gfaABC1D-Ezr-BioID2-HA

    Plasmid

    #176744

    Purpose

    Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Ezr-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB508-cTP-sfGFP

    Plasmid

    #213863

    Purpose

    Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused sfGFP
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEG03

    Plasmid

    #154138

    Purpose

    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

    Depositor

    Kang Zhou

    Article

    Panda et al Biochemical Engineering Journal

    Insert

    fucO, aldA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P_gyrA

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS_TXS

    Plasmid

    #163839

    Purpose

    iPTG inducible expression of taxadiene synthase and geranylgeranyl diphosphate synthase

    Depositor

    Jerome Fox

    Insert

    taxadiene synthase (61-862)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Trc

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. His6-TEV-Venus-pBluescript

    Plasmid

    #166756

    Purpose

    For purification of Venus fluorophore

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Venus
    

    Use

    Tags

    6x Histidine tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Use

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-luxOFP

    Plasmid

    #229945

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

    Insert

    luxOFP
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET26b_Nb.b201

    Plasmid

    #131404

    Purpose

    E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody b201
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

    Plasmid

    #159698

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pet30b-5M SrtA

    Plasmid

    #51140

    Purpose

    expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Article

    Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

    Insert

    S.aureus SrtA 5M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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