31,059 results

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pKM201

Plasmid

#13078

Depositor

Kenan Murphy

Article

Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

Insert

Ptac-gam-bet-exo operon

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pGS140_6xHis-EGFP-I3-01

Plasmid

#211889

Purpose

Bacterial expression of I3-01

Depositor

Iain Cheeseman

Insert

I3-01

Expression

Bacterial

Mutation

N/A

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Article

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Tags

His

Expression

Bacterial

Promoter

T5/Lac

Available Since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pSET-dCas9

Plasmid

#110183

Purpose

Expression of the dCas9 gene in Streptomyces

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Insert

dCas9

Use

CRISPR; I

Expression

Bacterial

Mutation

D10A, H840A

Promoter

ermE*p

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-hM4D(Gi)

Plasmid

#45548

Purpose

Expresses hM4D (Gi) neuronal inhibitor

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

hM4D (Gi)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,4,5)P3 Probe

Plasmid

#211514

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BTK1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT107

Plasmid

#85525

Purpose

Repressilator with triple reporter

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI
mKate2

Use

Synthetic Biology

Tags

ssrA

Expression

Bacterial

Available Since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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His6-SpyCatcher-mi3

Plasmid

#113043

Purpose

Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

His6-SpyCatcher-mi3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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PT7lacO-RF1-His6

Plasmid

#224372

Purpose

Express RF1 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

RF1 (prfA E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-Cdc34a

Plasmid

#18674

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Cdc34A (CDC34 Human)

Tags

His

Expression

Bacterial

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
MAC-tag-N

Plasmid

#108078

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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bZiPro

Plasmid

#86846

Purpose

express NS2B-NS3 protease in E.coli

Depositor

Dahai Luo

Article

Zhang et al Science. 2016 Dec 8. pii: aai9309.

Inserts

NS3 1-177
NS2B45-96

Tags

a hexahistidine tag and a thrombin cleavage site

Expression

Bacterial

Mutation

NS2B cofactor region (residues 45–96) and NS3 pro…

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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PVS10

Plasmid

#104398

Purpose

polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Article

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP1346

Plasmid

#119274

Purpose

knockdown folA in K. pneumoniae

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA folA (K. pneumoniae)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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ClpP-his

Plasmid

#22144

Depositor

Robert Sauer

Article

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

ClpP-His6

Tags

his

Expression

Bacterial

Mutation

C-terminal His tag

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_snap_his

Plasmid

#210407

Purpose

For expression of VHH with C-terminal snap protein and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pQE 80L Kan T7WT

Plasmid

#102790

Purpose

Expresses T7 RNA polymerase

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

T7 RNA polymerase

Expression

Bacterial

Available Since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCYP01

Plasmid

#212834

Purpose

Expresses the Rhizobium aethiopicum LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2T

Plasmid

#15808

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2T (UBE2T Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pBWB162

Plasmid

#140634

Purpose

pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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