33,902 results
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Plasmid#158065PurposeExpressing evolved CasTA of dCas9 fused AsiA variant 2.1DepositorInsertdCas9-AsiA_m2.1
ExpressionBacterialPromoterTetOAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαs-LgB113 (GNAS Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB591
Plasmid#141096PurposeE. coli-Lactobacilli shuttle vector for recombineering template cloningDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046785
Plasmid#145684PurposeBacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pF4Ag NanoLuc (ATG-42)
Plasmid#137777PurposeExpresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanoLuc (Nluc)
ExpressionBacterial and MammalianMutationNanoLuc contains 16 amino acid changes compared t…PromoterT7 and CMVAvailable sinceMarch 27, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pTOX4
Plasmid#127449PurposeEncodes YhaV toxin and amilCP chromoprotein. CAM-RDepositorInsertsyhaV toxin
amilCP chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
SuperNova Green/ pRSETB
Plasmid#124839Purposebacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent proteinDepositorInsertSuperNova Green
TagsNo tagExpressionBacterialAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)
Plasmid#114070PurposeT7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tagDepositorInsertbacteria codon optimized LbCas12a with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorPublicationTypeEmpty backboneUseCre/Lox; Herpesvirus cloningExpressionBacterialAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLBH559_Tet-HisCas14a1Locus
Plasmid#112502PurposeExpresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14a1
UseCRISPRTags6xHis-TEVExpressionBacterialPromoterTetAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI
Plasmid#115219PurposeExpresses PspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPspCas13
TagsSUMOExpressionBacterialAvailable sinceSept. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
T777T
Plasmid#113082PurposeEnhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.DepositorTypeEmpty backboneUseRNAiExpressionBacterialAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAJM.677
Plasmid#108530PurposeAraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.DepositorInsertAraCAM + AraE + PBAD-YFP
ExpressionBacterialMutationAraC: L133F E165L E169G C280LAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.011
Plasmid#108529PurposeTetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.DepositorInsertTetR + PTet*-YFP
ExpressionBacterialAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgRNAtet-lvaA
Plasmid#106397PurposeGuide RNA plasmid targeting lvaA on BBR1-UP originDepositorInsertsgRNA towards lvaA in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
AsCpf1-2NLS
Plasmid#102565Purposebacterial expression of AsCpf1-2NLSDepositorInsertAsCpf1
Tags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…ExpressionBacterialPromoterPA1Available sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMV762-Peredox-mCherry
Plasmid#90217PurposeFluorescent reporter for NADH:NAD+ ratios in Mycobacteria.DepositorInsertCodon Optimized Peredox-mCherry
UseShuttle vectorExpressionBacterialPromotergroELAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-NLS-Geo_st
Plasmid#87703PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLSDepositorInsertGeoCas9
Tags10xHis-MBP-TEVExpressionBacterialAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by