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pET StrepII TEV LIC cloning vector (2R-T)

Plasmid

#29717

Depositor

Scott Gradia

Type

Empty backbone

Tags

StrepII-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pGex-4T-2_GATE-16

Plasmid

#73518

Purpose

For recombinant expression of human GATE-16/GABARAPL2 in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

GATE-16 (GABARAPL2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pF

Plasmid

#42519

Depositor

Terry Magnuson

Article

Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

Type

Empty backbone

Use

Unspecified; Cloning

Expression

Bacterial

Promoter

T7, T3

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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B7-5

Plasmid

#111493

Purpose

translational initiation factor 1

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 1

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-His6

Plasmid

#51532

Purpose

Express His6-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pZE31ms2

Plasmid

#25860

Depositor

Philippe Cluzel

Article

Le et al Proc Natl Acad Sci U S A. 2005 Jun 28. 1

Insert

ms2 binding site (x2)

Expression

Bacterial

Mutation

none

Available Since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pET21a-C2m2-mKate

Plasmid

#208765

Purpose

To express C2m2-mKate in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-mKate (Mfge8 Mouse)

Tags

mKate

Expression

Bacterial

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

T7 promoter

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pInh

Plasmid

#199998

Purpose

Inhibitor of transposase and integrase

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Landing Pads

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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C332

Plasmid

#141124

Purpose

CinI expressing plasmid

Depositor

Matthew Bennett

Article

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Insert

CinI (cinI )

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

engineered lac promoter

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDL14

Plasmid

#106901

Purpose

pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

Depositor

Sergey Sineoky

Article

Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

Inserts

gam
bet
mutS
lacI
rhaR
rhaS

Expression

Bacterial

Mutation

Lysine 622 was replaced by Alanine

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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