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pTriEx-mVenus-Zdk1-RhoA Q63L

Plasmid

#81046

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-PLtetO-1-UTR1-TetR-deGFP-T500

Plasmid

#45774

Depositor

Richard Murray

Insert

tetR-deGFP

Use

Synthetic Biology

Tags

Fusion protein of TetR and deGFP, with glycine-se…

Expression

Bacterial

Promoter

PLtetO-1

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SH2-B-SH2

Plasmid

#46479

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B (SH2B1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Ana GvpC-SpyTag-His in pET28a

Plasmid

#85737

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (fused to SpyTag and His-tagged)

Tags

His-tag and SpyTag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-TelgRNA_PuroR

Plasmid

#84782

Purpose

Expresses telomere-specific sgRNA.

Depositor

Heinrich Leonhardt

Article

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Insert

telomere-specific sgRNA

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_SNX18-1/1

Plasmid

#91369

Purpose

Protein expression and purification of human SH3 domain construct SNX18-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX18-1/1 (SNX18 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPTD-DRBD

Plasmid

#35622

Depositor

Steven Dowdy

Article

Eguchi et al Nat Biotechnol. 2009 Jun;27(6):567-71. E

Insert

PKR DRBD-1

Tags

6xHIS, HA, and TAT

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pTriex-mVenus-PI-RhoA(Q63L)

Plasmid

#86978

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
RhoA (RHOA Human)

Tags

6xHis and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Ataxin3Q22

Plasmid

#22117

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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p_SNIPR_target_WT

Plasmid

#139465

Purpose

T7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensor

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

Target_WT

Expression

Bacterial

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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