31,059 results
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Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLumi-LgBiT
Plasmid#188319PurposeExpression of LgBiT via sigma 70 promoter in E. coliDepositorInsertLgBiT
UseLuciferase and Synthetic BiologyExpressionBacterialPromotersigma 70 promoterAvailable SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Antares
Plasmid#74279PurposeConstitutive expression of Antares in bacteriaDepositorInsertAntares
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-E2-Crimson
Plasmid#111252PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with E2-CrimsonDepositorInsertE2-Crimson
ExpressionBacterialPromoterPcAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TetR/pTet-Tnpase (camR)
Plasmid#213910PurposeATc-inducible himar1C9 transposase expression - cam resistanceDepositorInsertTetR/ptet ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2
Plasmid#213907PurposeBarcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
araBAD-BCD20-ClpP-BCD17-ClpX
Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorExpressionBacterialPromoteraraBADAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSK9065
Plasmid#84345Purposetightly controlled expression of mRFP fusion proteins in S. aureusDepositorTypeEmpty backboneTagsmRFPmarsExpressionBacterialPromoterPxyl/tetAvailable SinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-HpaBC
Plasmid#71260PurposeExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6DepositorInsertsHpaB
HpaC
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-cys-His6-OtUBD
Plasmid#190091PurposeExpresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD
Tags6xHisExpressionBacterialAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC574
Plasmid#127975PurposeAMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInserthygB (hygromycin resistance marker)
UseFungal expressionExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH624-ssr
Plasmid#199241PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertsingle-stranded DNA recombineering
ExpressionBacterialPromoterlacIq-PtrcAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (2M-T)
Plasmid#29708DepositorTypeEmpty backboneTags6xHis, MBP, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNP1954
Plasmid#222730PurposepNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)DepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD864-LacZ
Plasmid#109380PurposeRhamnose inducible LacZ expression. Used to test burden response in E. coliDepositorInsertpRHAM-LacZ
ExpressionBacterialAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pS2513·PHP
Plasmid#122590PurposeConstitutive expressed pHiorine2 for pH quan. in gram-negative cellsDepositorInsertphIorine2
UseSynthetic BiologyExpressionBacterialPromoterPem7Available SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM643
Plasmid#112533PurposepZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
ExpressionBacterialMutationA17PPromoterJ23107, PL(HrtO), and ProDAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJWV102-PL-dCas9
Plasmid#85588PurposeIntegrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9spDepositorInsertdCas9sp
UseCRISPRExpressionBacterialPromoterPLspAvailable SinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
SL-SunTag
Plasmid#196919PurposeThe SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loopDepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-slpGFP
Plasmid#27171DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
ExpressionBacterialAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only