30,260 results

1. pET24a-VHH-std

   Plasmid

   #109417

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGT-Kh2

   Plasmid

   #122587

   Purpose

   MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. NW30

   Plasmid

   #133444

   Purpose

   Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW30
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28c-F30-Broccoli

   Plasmid

   #66788

   Purpose

   Expresses Broccoli in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

   Insert

   Broccoli in the F30 scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. His-MBP_G4G6 in pRSF

   Plasmid

   #188454

   Purpose

   Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   G4G6 (GSN Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PIA586

   Plasmid

   #104399

   Purpose

   vector for expression of single E.coli RNAP subunit

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoD
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBud-EGFP

   Plasmid

   #23027

   Depositor

   Andrea LeBlanc

   Article

   Jodoin et al PLoS One. 2009 . 4(8):e6647.

   Insert

   EGFP (eGFP )
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease

   Plasmid

   #228637

   Purpose

   Bacterial expression of the Coxsackievirus 16 3C protease

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Coxsackievirus 16 3C protease
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. AB-GFP

   Plasmid

   #72203

   Purpose

   screen AB42 variants for aggregation

   Depositor

   Michael Hecht

   Article

   Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

   Insert

   AB42 (APP Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mOrange2-pBAD

    Plasmid

    #54531

    Purpose

    Localization: Protein Expression Vector , Excitation: 549, Emission: 565

    Depositor

    Nathan Shaner, Michael Davidson, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis and mOrange2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pE-SUMO CRYGD

    Plasmid

    #80755

    Purpose

    Expression of human gamma D-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGD (CRYGD Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEx-4T-1-YTHDF1

    Plasmid

    #70087

    Purpose

    Encode YTHDF1 with N-terminal GST tag for E. coli expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC18T-mini-Tn7T-hph-dCas9

    Plasmid

    #218371

    Purpose

    Expression of dCas9 for CRISPR-interference

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtetA

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWH1266-Apra-sgRNA

    Plasmid

    #218372

    Purpose

    sgRNA expression plasmid for CRISPR-intereference

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Insert

    sgRNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHybrid-Electra1

    Plasmid

    #179474

    Purpose

    Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Inserts

    Electra1
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-Bcatfl

    Plasmid

    #24193

    Depositor

    David Rimm

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET-15b-TEV-C9R

    Plasmid

    #118754

    Purpose

    TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

    Depositor

    Yutaka Kuroda

    Article

    Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

    Insert

    Tobacco Etch Virus protease (NEWENTRY Synthetic)
    

    Use

    Tags

    C9R and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBADHisB-Sumo-mBaoJin

    Plasmid

    #229580

    Purpose

    Expresses mBaoJin in bacterial cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX4T-hLIG3

    Plasmid

    #81055

    Purpose

    bacterial expression of human LIG3

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    DNA ligase 3 (LIG3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge