30,738 results

1. pKL002

   Plasmid

   #98921

   Purpose

   pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

   Depositor

   Joel Kralj

   Article

   Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

   Inserts

   PROPS
   GCaMP6f
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD_His B_QuasAr2

   Plasmid

   #64134

   Purpose

   Fluorescent reporter of membrane voltage with improved sensitivity

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr2
   

   Expression

   Bacterial

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGJJ045

   Plasmid

   #183621

   Purpose

   abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

   Depositor

   Ben Lehner

   Article

   Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PS6K-mcherry-iATPsnFR1.1

   Plasmid

   #227238

   Purpose

   Inducible ATP sensor for E. coli

   Depositor

   Fuzhong Zhang

   Article

   Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

   Insert

   iATPsnFR1.1
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T-Gm-eyfp

   Plasmid

   #65031

   Purpose

   GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   eyfp
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pExp-Sumo

   Plasmid

   #112574

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8His and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBR322-PVCpnf18-21

   Plasmid

   #198273

   Purpose

   Contains only regulatory genes; for generating empty PVCs

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf18-21
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSKA413

   Plasmid

   #80381

   Purpose

   Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

   Depositor

   Mustafa Khammash

   Article

   Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

   Inserts

   ccaS (complement)
   ccaR (complement)
   gfpmut3
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   FLAG tag

   Expression

   Bacterial

   Promoter

   ccaR and cpcG2Δ59

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINB-OTUB1*

   Plasmid

   #65441

   Purpose

   Expresses activated OTUB1 in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Inserts

   UbcH5b (UBE2D2 Human)
   otubain-1 (OTUB1 )
   

   Tags

   His-3C

   Expression

   Bacterial

   Mutation

   Active site cysteine mutated to alanine

   Promoter

   T7

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pAmCyan1-C1-ADCY6

    Plasmid

    #113905

    Purpose

    N-terminal Cyan Fluorescent tag in Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 (ADCY6 Human)
    

    Tags

    Cyan

    Expression

    Bacterial and Mammalian

    Promoter

    pCMV

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ404-DogCatcher-sfGFP

    Plasmid

    #171930

    Purpose

    Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    DogCatcher-sfGFP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

    Plasmid

    #185713

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed W403 and F753 to A

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBR322-Pdp1_NTD-Cas9

    Plasmid

    #198275

    Purpose

    For loading SpCas9 into PVCs; also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-Cas9
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid

    #197572

    Purpose

    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    3TAG and 45TAG

    Promoter

    araC and lpp

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE-Tn7-144

    Plasmid

    #118560

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3990 pET ubcH8

    Plasmid

    #8650

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

    Insert

    UbcH8 (UBE2L6 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pGEXTK-Pak1 70-117

    Plasmid

    #12217

    Depositor

    Jonathan Chernoff

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    PBD (p21-binding domain), aa 70-117

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOSIP-KL (KanR, Lambda)

    Plasmid

    #45984

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBZS-M.CviPII (NDel15)

    Plasmid

    #198356

    Purpose

    Express M.CviPII (NDel15) in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    M.CviPII
    

    Expression

    Bacterial

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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