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pET151-D-TOPO-PARP14-SΔK

Plasmid

#216658

Purpose

Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

Depositor

Herwig Schüler

Article

Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

Insert

PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

Deletion starting from Q1390 to D1448 and substit…

Promoter

T7

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57_beta-globin-5'UTR_Nanoluc

Plasmid

#219989

Purpose

betaglobin-5'UTR-NanoLuc mRNA synthesis

Depositor

Simon Alberti

Insert

betaglobin-5'UTR-NanoLuc (HBB Human)

Expression

Bacterial

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pETTEV-SENP8

Plasmid

#196361

Purpose

protein expression of the His-tagged full length protein

Depositor

Stefan Knapp

Insert

SENP8/NEDP1

Tags

His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-VAMP3

Plasmid

#135552

Purpose

Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP3

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 TIM8,13

Plasmid

#170282

Purpose

Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

Insert

yeast Tim8 and Tim13 (TIM8 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag on Tim13 (on bac…

Expression

Bacterial

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR6A1_Hormone-recep

Plasmid

#109945

Purpose

Protein expression and purification of NR6A1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR6A1_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.02

Plasmid

#131372

Purpose

expression of MBP:PGL-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pgl-1 (pgl-1 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Expression

Bacterial

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-EZH2-T487A mutant

Plasmid

#28061

Depositor

Mien-Chie Hung

Article

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Tags

GST

Expression

Bacterial

Mutation

T487A

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only