4,931 results

1. pAFMW-Nbr[R280A,K284A]

   Plasmid

   #188598

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   R280A [CGA=>GCA], K284A [AAG=> GCG]

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINF Ta-sro1 PARP

   Plasmid

   #192549

   Purpose

   E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
   

   Tags

   His6, 3C protease cleavage site

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   T7 promoter

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5-STABLE2-neo-RvCAHS3

   Plasmid

   #192472

   Purpose

   Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS3
   

   Tags

   T2A-EGFP-T2A-neoR

   Expression

   Insect

   Promoter

   Ac5 promoter

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1

   Plasmid

   #192470

   Purpose

   Transient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS8
   

   Tags

   AcGFP1

   Expression

   Insect

   Promoter

   Ac5 promoter

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1

   Plasmid

   #192469

   Purpose

   Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS3
   

   Tags

   AcGFP1

   Expression

   Insect

   Promoter

   Ac5 promoter

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII

   Plasmid

   #192705

   Purpose

   Plasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728

   Depositor

   Sascha Martens

   Inserts

   ATG5 (ATG5 Human)
   ATG7 (ATG7 Human)
   ATG10 (ATG10 Human)
   ATG12 (ATG12 Human)
   ATG16L1
   

   Tags

   -GFP-TEV-StrepII and 10x-His-TEV

   Expression

   Insect

   Mutation

   F32A-I35A-I36A

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAFMW-Nbr[R272A,K276A, R280A,K284A]

   Plasmid

   #188599

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PRE-Hsp70BbCas9_1.0

   Plasmid

   #190795

   Purpose

   PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   PRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PALI1_PIR_pFB1

   Plasmid

   #191249

   Purpose

   Expresses PALI1 from insect cells

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

   Insert

   LCoR
   

   Tags

   MBP

   Expression

   Insect

   Mutation

   NA

   Promoter

   Polyhedrin

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MON +T111H

    Plasmid

    #187432

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed threonine at 117 to histidine

    Promoter

    PH and p10

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAFMW-Nbr[K216E]

    Plasmid

    #188600

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K216E [AAA=> GAA]

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAFMW-Nbr[C399-625 CM]

    Plasmid

    #188588

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler C-terminal domain, catalytic mutant
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    D435A [GAT=>GCC]; E435A [GAA=>GCC]

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAFMW-Nbr[C399-625]-Gwpep(Mm)

    Plasmid

    #188589

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
    

    Tags

    FLAG-Myc and GWpep mm

    Expression

    Insect

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAFMW-Nbr[K276A]

    Plasmid

    #188597

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K276A [AAG=>GCG]

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAFMW-Nbr[C399-625]-Gwpep(Dm)

    Plasmid

    #188590

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
    

    Tags

    FLAG-Myc and Gwpep dm

    Expression

    Insect

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAFMW-Gwpep(Dm)-Nbr[C399-625]

    Plasmid

    #188592

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
    

    Tags

    FLAG-Myc-Gwpep dm

    Expression

    Insect

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAFMW-Gwpep(Mm)-Nbr[C399-625]

    Plasmid

    #188591

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
    

    Tags

    FLAG-Myc-Gwpep mm

    Expression

    Insect

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAFMW-Nbr[R272A,K276A]

    Plasmid

    #188596

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG], K276A [AAG=>GCG]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAFMW-Nbr[K284A]

    Plasmid

    #188595

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K284A [AAG=> GCG]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAFMW-Nbr[R272A]

    Plasmid

    #188594

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAFMW-Nbr[K187A]

    Plasmid

    #188593

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K187A [AAA=> GCA]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAFMW-Nbr-CM

    Plasmid

    #188585

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler Catalytic mutant
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG; D435A [GAT=>GCC]; E435A [GA…

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAFMW-Nbr

    Plasmid

    #188584

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAFMW-Nbr[N1-383]

    Plasmid

    #188586

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler N-terminal domain
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAFMW-Nbr[C399-625]

    Plasmid

    #188587

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler C-terminal domain
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pACE-K2P4.1-StrepTagII

    Plasmid

    #191474

    Purpose

    Expresses K2P4.1 with C-terminal StrepTagII

    Depositor

    Arthur Laganowsky

    Article

    Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

    Insert

    K2P4.1 (KCNK4 Human)
    

    Expression

    Insect

    Mutation

    N104Q N108Q

    Promoter

    polH

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. OA-1127B

    Plasmid

    #190997

    Purpose

    Expresses arrays of gRNA targeting rel1 promoter under U6b/c promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    U6b:sgRNArel1A1 - U6c:sgRNArel1A2 - U6b:sgRNArel1A3 - U6c:sgRNArel1A4
    

    Expression

    Insect

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KEE

    Plasmid

    #187419

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Promoter

    PH and p10

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p1X-UASTattB

    Plasmid

    #189861

    Purpose

    Fly cloning and transformation

    Depositor

    Jung Hwan Kim

    Article

    Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBac-M13-6His-KIF1C_R169W-GFP

    Plasmid

    #185979

    Purpose

    Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    6xHis and eGFP

    Expression

    Insect

    Mutation

    R169W - mutation causing HSP (SPG58)

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TEG +H122D

    Plasmid

    #187434

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed histidine at 128 to aspartic acid

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pACE-GIRK2-mC-S

    Plasmid

    #172428

    Purpose

    Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK2 (KCNJ6 Human)
    

    Tags

    mCherry Streptag II

    Expression

    Insect

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FER +H111E

    Plasmid

    #187429

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed histidine at 117 to glutamic acid

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. KEE +E111H

    Plasmid

    #187431

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed glutamic acid at 117 to histidine

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SNG +R111Q

    Plasmid

    #187437

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed arginine at 117 to glutamine

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FER +H122D

    Plasmid

    #187428

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed histidine at 128 to aspartic acid

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MON +N122H

    Plasmid

    #187433

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed asparagine at 128 to histidine

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. OST +Q111R

    Plasmid

    #187436

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed glutamine at 116 to arginine

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FER +H111T

    Plasmid

    #187430

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed histidine at 117 to threonine

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. RAT +D122H

    Plasmid

    #187426

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Expression

    Insect

    Mutation

    changed aspartic acid at 129 for histidine

    Promoter

    PH and p10

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge