4,931 results

1. pAc5.1B-EGFP-DmTral-R42EF44A_C

   Plasmid

   #146061

   Purpose

   Insect Expression of DmTral-R42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42EF44A (tral Fly)
   

   Expression

   Insect

   Mutation

   R42E, F44A. Additionally, two silent mutations A1…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

   Plasmid

   #146049

   Purpose

   Insect Expression of DmTral-LSmR21EE23KS13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KS13AI15A (tral Fly)
   

   Expression

   Insect

   Mutation

   S13A, I15A, R21E, E23K, one silent mutation A198C…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

   Plasmid

   #146039

   Purpose

   Insect Expression of DmTral-FDF-ADA

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmTral-FDF-ADA (tral Fly)
   

   Expression

   Insect

   Mutation

   FDF to ADA (407-409) mutation. Additionally, thre…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACEBac1-Q22YU3 del C3-2xStrep

   Plasmid

   #170316

   Purpose

   Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Q22YU3 C-terminal truncation (Delta-C3)
   

   Tags

   Strep-Tag II

   Expression

   Insect

   Mutation

   Deletion of C-terminal C3-finger

   Promoter

   polyhedrin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACEBac1-Q22MS1-2xStrep

   Plasmid

   #170314

   Purpose

   Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag II

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Q22MS1
   

   Tags

   Strep-Tag II

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1C-5Box-7-Fluc-STOP-CG10011-SV40_D

   Plasmid

   #146156

   Purpose

   Insect Expression of 5BoxB-7-Fluc-CG10011-3UTR

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al RNA. 2009 Jan . 15(1):21-32.

   Insert

   5BoxB-7-Fluc-CG10011-3UTR
   

   Expression

   Insect

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-EGFP-DmMe31B_268-459_D

   Plasmid

   #146119

   Purpose

   Insect Expression of DmMe31B_268-459

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmMe31B_268-459 (me31B Fly)
   

   Expression

   Insect

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-DmPABPC1-V5His6_D

   Plasmid

   #146095

   Purpose

   Insect Expression of DmPABPC1

   Depositor

   Elisa Izaurralde

   Insert

   DmPABPC1 (pAbp Fly)
   

   Expression

   Insect

   Mutation

   seven silent mutations compared to the sequence g…

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVMG0193_Cq-Rpl40-Cas9

   Plasmid

   #169346

   Purpose

   Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

   Depositor

   Valentino Gantz

   Article

   Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

   Insert

   pVMG0193_Cq-Rpl40-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   CPIJ002413

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVMG0217_Cq-U6-2b_2xBbsI-gRNA

    Plasmid

    #169339

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0217_Cq-U6-2b_2xBbsI-gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ039728

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

    Plasmid

    #146013

    Purpose

    Insect Expression of DmSmaug_763-999

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug_763-999 (smg Fly)
    

    Expression

    Insect

    Mutation

    2 silent mutations and a deletion of the AA 961-9…

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-EGFP-DmTral-R42E_B

    Plasmid

    #145988

    Purpose

    Insect Expression of DmTral-R42E

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-R42E (tral Fly)
    

    Expression

    Insect

    Mutation

    two silent mutations A198C, A387G and a mutation …

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

    Plasmid

    #146001

    Purpose

    Insect Expression of DmSmaug_1-762

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug_1-762 (smg Fly)
    

    Tags

    V5-6xHis

    Expression

    Insect

    Mutation

    NP_523987.1, 7 silent mutations compared to the s…

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-DmZuc_B

    Plasmid

    #145949

    Purpose

    Insect Expression of DmZuc

    Depositor

    Elisa Izaurralde

    Insert

    DmZuc (zuc Fly)
    

    Expression

    Insect

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmExu_B

    Plasmid

    #145939

    Purpose

    Insect Expression of DmExu

    Depositor

    Elisa Izaurralde

    Insert

    DmExu (exu Fly)
    

    Expression

    Insect

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmAGO1-F629V_A

    Plasmid

    #145898

    Purpose

    Insect Expression of DmAGO1-F629V

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    DmAGO1-F629V (AGO1 Fly)
    

    Expression

    Insect

    Mutation

    V315A, F629V, H913R - variation with 2 non silent…

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-DmLSm3_A

    Plasmid

    #145888

    Purpose

    Insect Expression of DmLSm3

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

    Insert

    DmLSm3 (LSm3 Fly)
    

    Expression

    Insect

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmDCP2_209-791_A

    Plasmid

    #145848

    Purpose

    Insect Expression of DmDCP2_209-791

    Depositor

    Elisa Izaurralde

    Insert

    DmDCP2_209-791 (DCP2 Fly)
    

    Expression

    Insect

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac1_GI.1_A37C-A44C

    Plasmid

    #162582

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Alanine 37 changed to Cysteine and Alanine 44 cha…

    Promoter

    polyhedring

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac1_GI.1_A37I-A44L

    Plasmid

    #162583

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    A37I and A44L

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFastBac1_GI.1_Q141V-P221L

    Plasmid

    #162584

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Glutamine 141 changed to Valine and Proline 221 c…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFastBac1_GI.1_L144C-P221C

    Plasmid

    #162585

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Leucine 144 changed to Cysteine and Proline 221 c…

    Promoter

    polyhderin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFastBac1_GI.1_G131C-N172C

    Plasmid

    #162586

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Glycine 131 changed to Cysteine and Asparagine 17…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFastBac1_GI.1_N167C-L169C

    Plasmid

    #162587

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Asparagine 167 changed to Cysteine and Leucine 16…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 1026L=U6-3-gRNA#1[Pol-γ35]

    Plasmid

    #159674

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#1[Pol-γ35]
    

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 1026M=U6-3-gRNA#2[Pol-γ35]

    Plasmid

    #159675

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#2[Pol-γ35]
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KIF13B

    Plasmid

    #163907

    Purpose

    Full length mouse KIF13B for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KIF13B 1-584

    Plasmid

    #163908

    Purpose

    Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mWasabi-ADAP1

    Plasmid

    #163906

    Purpose

    Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Tags

    mWasabi fluorescent tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

    Plasmid

    #163927

    Purpose

    Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

    Plasmid

    #163925

    Purpose

    Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
    

    Use

    Cre/Lox

    Tags

    tagBFP

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDest-639 (attR4-attR2)

    Plasmid

    #161880

    Purpose

    Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDest-624 (attR4-attR2)

    Plasmid

    #161879

    Purpose

    Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 58 pLIB_SNAP

    Plasmid

    #160307

    Purpose

    N-term 3C cleavable SNAP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    12xHis and SNAP

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAc-­His-­zfKitlgb

    Plasmid

    #140293

    Purpose

    Express soluble His-tagged zebrafish Kitlgb in insect cells

    Depositor

    Petr Bartunek

    Article

    Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

    Insert

    Kitlgb (kitlgb Zebrafish)
    

    Tags

    6x His

    Expression

    Insect

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc-­His-­zfKitlga

    Plasmid

    #140292

    Purpose

    Express soluble His-tagged zebrafish Kitlga in insect cells

    Depositor

    Petr Bartunek

    Article

    Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

    Insert

    Kitlga (kitlga Zebrafish)
    

    Tags

    6x His

    Expression

    Insect

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 48 pLIB_C_GST

    Plasmid

    #160273

    Purpose

    C-term uncleavable GST tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    GST

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 49 pLIB_3C_C_GST

    Plasmid

    #160274

    Purpose

    C-term 3C cleavable GST tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    GST

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 908I

    Plasmid

    #160241

    Purpose

    VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Van Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Tags

    T2A GFP

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 907F1

    Plasmid

    #160233

    Purpose

    FlightN-VanR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    VanR
    Flightin promoter
    

    Tags

    VP16

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge