4,908 results

1. pUAS-syb:spGFP1–10

   Plasmid

   #73698

   Purpose

   Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

   Depositor

   Marco Gallio

   Article

   Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

   Insert

   Neuronal-synaptobrevin:spGFP1–10 chimera
   

   Use

   Drosophila

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJFRC2-10XUAS-IVS-mCD8::GFP

   Plasmid

   #26214

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   mCD8::GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDR125

   Plasmid

   #37150

   Depositor

   Dale Ramsden

   Insert

   lig4 (LIG4 Human)
   

   Use

   Tags

   H6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pCasper4-g-osk-TRICK

   Plasmid

   #71244

   Purpose

   oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   oskar (osk Fly)
   

   Use

   P element-mediated germline transformation

   Tags

   12xPP7 and 6xMS2

   Expression

   Insect

   Mutation

   Promoter

   osk

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOmnibac_ZZ_linker_K963_GFP_SNAP

   Plasmid

   #159727

   Purpose

   Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
   

   Use

   Tags

   GFP and SNAP tags and ZZ affinity tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAC-ECFP-15xQUAS_TATA-SV40

   Plasmid

   #104875

   Purpose

   piggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformation

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. dVGlut-Gal80

   Plasmid

   #52555

   Purpose

   Expresses Gal80 under vesicular glutamate transporter promoter

   Depositor

   Leslie Vosshall

   Insert

   VGlut-Gal80 (VGlut, GAL80 Budding Yeast, Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila Synthetic Core Promoter

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac-M13-6His-KIF1C-GFP

   Plasmid

   #130975

   Purpose

   Expression of 6His-KIF1C-GFP in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Use

   Tags

   6His and GFP

   Expression

   Insect

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   Polyhedrin

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 20XUAS IVS ChrimsonR mCherry_tr

   Plasmid

   #111546

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLIB_GST-TEV-SRC (Y530F)

    Plasmid

    #223743

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Src Kinase (SRC Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Y530F constitutively active mutant

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

    Plasmid

    #217512

    Purpose

    KD Recombinase dependent conditional smGdP-10XOllas cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
    

    Use

    Tags

    smGdP-10XOllas

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ac5-STABLE1-blast

    Plasmid

    #208033

    Purpose

    Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162383

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-Hrp48-ADARcd-V5

    Plasmid

    #81172

    Purpose

    identification of cell-specific targets of RNA binding proteins

    Depositor

    Michael Rosbash

    Article

    McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

    Insert

    Hrp48-ADARcd fusion (Hrb27C Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila metallothionein promoter

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJC-repo1.9kb-TALE4-VP64-P10

    Plasmid

    #104612

    Purpose

    Expresses TALE4 under the control of a 1.9kb repo enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.9kb repo enhancer element (repo Fly)
    

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc V5 dDispatched WT HA

    Plasmid

    #126405

    Purpose

    Expression of V5 dDispatched WT HA in Drosophila cells lines.

    Depositor

    Stacey Ogden

    Article

    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

    Insert

    d-Dispatched1 (disp Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac-hDicer

    Plasmid

    #89144

    Purpose

    Expression of hDicer in Sf9 Cells

    Depositor

    Jennifer Doudna

    Article

    MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

    Insert

    hDicer (DICER1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pE5n

    Plasmid

    #17459

    Purpose

    modified pENTR vector for N-terminal ECFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    ECFP

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. TH1204-pOCC120-FUS_opt(NheI)

    Plasmid

    #221892

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    MBP-PreScission and mGFP-PreScission-6xHis

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

    Plasmid

    #55220

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    His6 and MBP, Asn10

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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