5,019 results

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pQFBD

Plasmid

#165823

Purpose

QF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QFBD

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQFAD

Plasmid

#165824

Purpose

QF activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QFAD

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGGGSx4

Plasmid

#165807

Purpose

Flexible peptide linker of 4 repeats of GGGS, for assembly of TL1 to TL5 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GGGSx4

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pT2A

Plasmid

#165809

Purpose

Viral T2A linker element for assembly of TL3 to TL5 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

T2A

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGGSx4 NT

Plasmid

#165810

Purpose

Flexible peptide linker of 4 repeats of GGS, for assembly of N-terminal TL1 tags to TL3 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GGSx4 NT

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Promoterless TP10

Plasmid

#165787

Purpose

Drosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectors

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Promoterless T10

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pR20A02

Plasmid

#165791

Purpose

GB2.0 compatible FlyLight Enhancer element R20A02 in enhancer grammar

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

R20A02

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pR70B04

Plasmid

#165792

Purpose

GB2.0 compatible FlyLight Enhancer element R70B04 in enhancer grammar

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

R70B04

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pR76H03

Plasmid

#165793

Purpose

GB2.0 compatible FlyLight Enhancer element R76H03in enhancer grammar

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

R76H03

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMCS-TCCC

Plasmid

#165774

Purpose

Multiple cloning site part for enhancer cloning, requires minimal promoter element

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

MCS-TCCC

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pdSCP

Plasmid

#165776

Purpose

Minimal synthetic Drosophila core promoter, requires enhancer element for full expression

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

dSCP

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

Plasmid

#172914

Purpose

Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7c-T2A-tdTomato

Tags

6xHis and T2A-tdTomato

Expression

Insect

Promoter

hsp70

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

Plasmid

#172913

Purpose

Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7b-T2A-tdTomato

Tags

6xHis and T2A-tdTomato

Expression

Insect

Promoter

hsp70

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-Nb127D01-mCherry-KDEL

Plasmid

#171575

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Tags

GST tag and His tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pACEBac1-Q22YU3-2xStrep

Plasmid

#170315

Purpose

Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag II

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22YU3 (TTHERM_00122270 Tetrahymena thermophila)

Tags

Strep-Tag II

Expression

Insect

Promoter

Polyhedrin

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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Mdn1-deltaC

Plasmid

#171603

Purpose

Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Tags

His6 tag

Expression

Insect

Mutation

C-terminal deletion; a.a. 1-3911 only

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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Mdn1-FL

Plasmid

#171602

Purpose

Expresses full length S. pombe Mdn1 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Tags

His6 tag

Expression

Insect

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral_394-543-V5His6_D

Plasmid

#146134

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Expression

Insect

Mutation

two silent mutations (nucleotides A198C, A387G) a…

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBelle_1-298_B

Plasmid

#145913

Purpose

Insect Expression of DmBelle_1-298

Depositor

Elisa Izaurralde

Insert

DmBelle_1-298 (bel Fly)

Expression

Insect

Mutation

del 158-160 (GGG)

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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