4,958 results

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pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

Plasmid

#163925

Purpose

Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor

Use

Cre/Lox

Tags

tagBFP

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-639 (attR4-attR2)

Plasmid

#161880

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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58 pLIB_SNAP

Plasmid

#160307

Purpose

N-term 3C cleavable SNAP tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

12xHis and SNAP

Expression

Insect

Promoter

polyhydrin

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfKitlgb

Plasmid

#140293

Purpose

Express soluble His-tagged zebrafish Kitlgb in insect cells

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlgb (kitlgb Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfKitlga

Plasmid

#140292

Purpose

Express soluble His-tagged zebrafish Kitlga in insect cells

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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48 pLIB_C_GST

Plasmid

#160273

Purpose

C-term uncleavable GST tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

GST

Expression

Insect

Promoter

polyhydrin

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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49 pLIB_3C_C_GST

Plasmid

#160274

Purpose

C-term 3C cleavable GST tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

GST

Expression

Insect

Promoter

polyhydrin

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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908I

Plasmid

#160241

Purpose

VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Van Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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907F1

Plasmid

#160233

Purpose

FlightN-VanR-VP16-3X-SV40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

VanR
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045B

Plasmid

#125004

Purpose

Expresses gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045D

Plasmid

#125006

Purpose

Expresses gRNAs targeting hedgehog and wingless

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

wg gRNA (wg Fly)

Expression

Insect

Promoter

u6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045E

Plasmid

#125007

Purpose

Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986C

Plasmid

#125000

Purpose

Expresses dCas9-VPR driven by the bottleneck promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

bottleneck

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986D

Plasmid

#125001

Purpose

Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Expression

Insect

Promoter

ubiquitin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986E

Plasmid

#125002

Purpose

Expresses dCas9-VP64 driven by the bottleneck promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Expression

Insect

Promoter

bottleneck

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045A

Plasmid

#125003

Purpose

Expresses gRNAs targeting hid and eve

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(1)-CRY2-mCherry

Plasmid

#160440

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(0)-CRY2-mCherry

Plasmid

#160439

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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R979-X57-008: Hs.KRAS4b(1-169)

Plasmid

#159690

Purpose

Baculovirus protein expression of KRAS4b (1-169)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-169) (KRAS Human)

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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