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Plasmid#132524PurposeFull length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragmentsDepositorInsertDYNC2H1 (DYNC2H1 Human)
UseTags8x His, Linker-TEV-linker-TEV, Precission cleavag…ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
52 pLIB_3C_C_STREP
Plasmid#160300PurposeC-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneUseTags2xStrepIIExpressionInsectMutationPromoterpolyhydrinAvailable sinceNov. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT Stat92E-GFP
Plasmid#126662PurposeInducible expression of Stat92E-GFP in S2 cellsDepositorInsertStat29E
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE1-puro
Plasmid#208031PurposeEnables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-mtdT-3XHA
Plasmid#24354DepositorInsertmtDT-3XHA
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
57F03_LexA-GAD
Plasmid#83256PurposeExpresses LexA-GAD under the control of 57F03 enhancerDepositorInsert57F03 enhancer
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
dBrainbow
Plasmid#29327DepositorInsertUAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pUAST-attb
Plasmid#188041PurposeExpresses CapChR2 in DrosophilaDepositorInsertCapChR2
UseTagsnoneExpressionInsectMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterUASAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
DPYSL4 (DPYSL4A-c003)
Plasmid#110263PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDPYSL4 (DPYSL4 Human)
UseTagsHis6-ZBasic-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-3C-FIP200-GFP
Plasmid#187832PurposePlasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445DepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and mGFPExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-Act5c
Plasmid#130278PurposeDrosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.DepositorInsertpCFD3-Act5c (Act5C Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pActPL-VP16AD
Plasmid#15305DepositorInsertVP16AD-ZIP+
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pR I-Ab alpha BirA
Plasmid#113638PurposeInsect cell expression vector for I-Ab alpha chain fused to BirA biotinylation siteDepositorInsertI-Ab alpha BirA (H2-Ab1 Mouse)
UseTagsBirA biotinylation signal site and fused to Fos-J…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S S730A
Plasmid#169946PurposePurification of catalytically-dead DPP9S from insect cellsDepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationS730APromoterpolyhedrinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDam-Myc-HP1
Plasmid#59221Purposeexpresses Dam-myc-HP1DepositorInsertHP1 (Su(var)205 Fly)
UseDamidTagsDam and mycExpressionInsectMutationPromoterDrosophila heat shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv::NG
Plasmid#138953PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutationPromoterAvailable sinceNov. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBac-DsRed-ORCO_9kbProm-QF2
Plasmid#104877PurposeAnopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoesDepositorInsertAgORCO-QF2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only