4,958 results
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Plasmid#63752PurposeExpresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only
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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10
Plasmid#218867PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8f
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationQ315LPromoterhsp70Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBac{Act88F-eGFP,attB,w+}
Plasmid#182198PurposeEGFP driven by Act88F enhancerDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2
Plasmid#165831PurposeEBFP2blue fluorescent protein CDSDepositorInsertEBFP2
ExpressionInsectAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3x127D01-DsRed
Plasmid#171579PurposeFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQUAST>stop>mCD8-GFP
Plasmid#24361DepositorInsertmCD8-GFP
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-TAK1-V5-His
Plasmid#63753PurposeExpresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2Available SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Fla
Plasmid#84866PurposeBaculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Fla
UseBaculovirusExpressionInsectPromoterPolyhedrinAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC292
Plasmid#118881Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
TagsmRuby3, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW-CD8-NbVHH05-GFP
Plasmid#171576PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembraneDepositorInsertCD8-NbVHH05-GFP (UBE2J1 Human)
TagsCD8 and GFPExpressionInsectPromoterfly actin5C promoterAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac (GST-SNAP-Shp2)
Plasmid#177897PurposeBaculo expression of GST tag fused to SNAP tag and human Shp2DepositorInsertShp2 (PTPN11 Human)
TagsTEV-GST-PreScission cut site-SNAP tagExpressionInsectPromoterPolyhedrinAvailable SinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAtx2_AE
Plasmid#148548PurposeInsect Expression of DmAtx2DepositorInsertDmAtx2 (Atx2 Fly)
ExpressionInsectMutationThree silent mutations and two deletions compared…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
QPRTA-c002
Plasmid#205115PurposeQPRT protein expressionDepositorAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCoHygro L16_shRNA of spliced Copia
Plasmid#71389Purposeknock down expression of spliced Copia in Drosophila melanogasterDepositorInsertCopia
ExpressionInsectPromoterU6Available SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
10XUAS-flp-DD
Plasmid#124777PurposeExpresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.DepositorInsertTMP-inducible destabilized FLP (FLP-DD)
TagsDDExpressionInsectAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VP64
Plasmid#78901PurposeExpresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertdCas9-VP64
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriex-mVenus-PI-RhoA(Q63L)
Plasmid#86978PurposePI(DM): Photo-activatable/light sensitiveDepositorInsertsTags6xHis and mVenusExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only