4,908 results

1. pBMPGAL4LWL

   Plasmid

   #26270

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   GAL4 (GAL4 Budding Yeast)
   

   Use

   Drosophila transgenesis

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

   Plasmid

   #159636

   Purpose

   Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

   Depositor

   Carolyn McBride

   Article

   Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

   Insert

   GCaMP6s
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xTRE DREaMR 3p3GFP

   Plasmid

   #182205

   Purpose

   3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.

   Depositor

   Craig Montell

   Article

   Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

   Insert

   rTetR-3XP3-GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. DmU6 3p3 GFP LgRNA

   Plasmid

   #182207

   Purpose

   For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila

   Depositor

   Craig Montell

   Article

   Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

   Insert

   3XP3-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUAST mito roGFP2-Grx1

   Plasmid

   #64995

   Purpose

   for expression of mitochondrial roGFP2-Grx1 with the Gal4 expression system in drosophila

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBlue-TAL

   Plasmid

   #49401

   Purpose

   The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

   Depositor

   Michal Zurovec

   Article

   Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. IL2 F42A pAcGP67a

   Plasmid

   #41807

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   IL2 (IL2 Human)
   

   Use

   Tags

   6x His

   Expression

   Insect

   Mutation

   Phenylalanine 42 to Alanine

   Promoter

   Polyhedron

   Available since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pDEST8_FLAGHA_FUS_HIS

   Plasmid

   #26375

   Depositor

   Thomas Tuschl

   Article

   Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   FLAG/HA and His

   Expression

   Insect

   Mutation

   delta 527 (STOP codon)

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pFasBac+GFP-CAMSAP3 CC+CKK

   Plasmid

   #59041

   Purpose

   for baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cells

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP3 CC+CKK domain (Camsap3 Mouse)
   

   Use

   Tags

   His and eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

    Plasmid

    #71805

    Purpose

    Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCaSpeR4 mito roGFP2-Orp1

    Plasmid

    #65001

    Purpose

    Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Fast-GCaMP-EF05

    Plasmid

    #52558

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF05
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed from GCaMP3: D397N/G398A/N399D

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pBPHLWL-GAL80

    Plasmid

    #26257

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIHEU_miniSOG2 T2A H3.3A-EGFP

    Plasmid

    #87411

    Purpose

    UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

    Insert

    mini singlet oxygen generator 2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. UAS-Syt::3xHalo7

    Plasmid

    #87649

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::3xHalo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::3xHalo7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC167

    Plasmid

    #118909

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC240

    Plasmid

    #118895

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease and mCherry, clea…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC8_GWs-Lox (VE5589)

    Plasmid

    #163769

    Purpose

    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    no insert
    

    Use

    Tags

    no tag

    Expression

    Insect

    Mutation

    Promoter

    PH

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWalium-ChtVis-Tomato

    Plasmid

    #67756

    Purpose

    Expresses a fluorescent chitin reporter under Gal4 control in Drosophila.

    Depositor

    Paul Adler

    Article

    Sobala et al Development. 2015 Nov 15;142(22):3974-81

    Insert

    dyl - chitin binding domain - tdTomato - dyl
    

    Use

    Tags

    td-tomato

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGS-CD64-2

    Plasmid

    #109191

    Purpose

    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    Full-length engineered CD64, derived from human CD64
    

    Use

    Tags

    Twin-Strep

    Expression

    Insect

    Mutation

    engineered for efficient expression, see publicat…

    Promoter

    Polyhedrin

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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