4,908 results

1. ABCB8 (ABCB8A-c016)

   Plasmid

   #110266

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ABCB8 (ABCB8 Human)
   

   Use

   Tags

   TEV-His10-Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hy_pMT Flag-Hel25E WT

   Plasmid

   #110123

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Oct. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hy_pMT Flag-Hel25E K91N

   Plasmid

   #110121

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   K91N mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Hy_pMT Flag-Hel25E D193E

   Plasmid

   #110120

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   D193E mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTIGER_tdTomato::3xFLAG::dCrk

   Plasmid

   #131138

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   3XFLAG and tdTomato

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAST-SIK3 T196E

   Plasmid

   #52975

   Purpose

   insect expression of SIK3 T196E constitutively active mutant

   Depositor

   Marc Montminy, Biao Wang

   Article

   Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

   Insert

   SIK3 (Sik3 Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   T196E constitutively active mutant

   Promoter

   Hsp70

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pACEBac1-beta-actin

   Plasmid

   #178076

   Purpose

   Insect cell expression of beta-actin

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   beta-actin (ACTB Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)

   Plasmid

   #159577

   Purpose

   Baculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540)

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-MBP-tev-Hs.NF1 ABC (2-1540) (NF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. R791-X15-636: His6-MBP-tev-Hs.NF1 DEF (1541-2818)

   Plasmid

   #159578

   Purpose

   Baculovirus protein expression of His6-MBP-tev-Hs.NF1 DEF (1541-2818)

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-MBP-tev-Hs.NF1 DEF (1541-2818) (NF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAS myc Gr66a

    Plasmid

    #26861

    Depositor

    Craig Montell

    Article

    Moon et al Curr Biol. 2006 Sep 19. 16(18):1812-7.

    Insert

    Gr66 gustatory receptor (Gr66a Fly)
    

    Use

    Tags

    myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pAc-sgRNA-Cas9.scr_2

    Plasmid

    #190704

    Purpose

    Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Scrambled sgRNA 2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAFMW-Nbr-D435A/E437A

    Plasmid

    #40829

    Purpose

    Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

    Depositor

    Phillip Zamore

    Article

    Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

    Insert

    Nbr D435A/E437A (Nbr Fly)
    

    Use

    Tags

    3xFLAG and 6xMyc

    Expression

    Insect

    Mutation

    D435A/E437A, part of the DEDD motif; mutations p…

    Promoter

    Actin5C

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pFastBac mPh1

    Plasmid

    #1970

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    mPh1 (Phc1 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pKSB-631 U6-sgRNA3/4 AACA GCTT

    Plasmid

    #173675

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUAST-SIK3 WT

    Plasmid

    #52972

    Purpose

    insect expression of SIK3 WT

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCaSpeR-act5cB-QF#7

    Plasmid

    #46125

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT Draper-mCherry

    Plasmid

    #126667

    Purpose

    Inducible expression of Draper-mCherry in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    C813Y- see depositor comments below

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCaSper4 cyto roGFP2-Orp1

    Plasmid

    #64998

    Purpose

    Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EKAR2G_design1_mTFP_105_Venus_157

    Plasmid

    #39819

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pXL-BACII-attPGAL4LWL

    Plasmid

    #26260

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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