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pPac0

Plasmid

#12094

Depositor

Robert Tjian

Article

Courey et al Cell. 1988 Dec 2. 55(5):887-98.

Type

Empty backbone

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

Plasmid

#169189

Purpose

Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

Insert

3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-His6

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Rab6

Plasmid

#31734

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab6 (Rab6 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pFastBac HTB DPP9S S730A

Plasmid

#169946

Purpose

Purification of catalytically-dead DPP9S from insect cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Mutation

S730A

Promoter

polyhedrin

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-GAL4DBD

Plasmid

#26256

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

ZIP- Gal4DBD (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pUASt-GBP-Trbo

Plasmid

#179996

Purpose

Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

GBP nanobody

Tags

Trbo

Expression

Insect

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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K20‐scFv‐pSMBP2

Plasmid

#159940

Purpose

To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

Depositor

Sandra Schmid

Article

Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

Insert

anti beta-1 integrin scFv

Tags

6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

Expression

Insect

Promoter

Polyhedrin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX

Plasmid

#138387

Purpose

Empty RMCE vector (phiC31 attB/loxP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

P5' FRT3 3xP3-Venus φC31-attB loxP FRT3 P3'

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINO_H11

Plasmid

#184277

Purpose

Expresses nanobody H11 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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OA-961B

Plasmid

#104967

Purpose

D. suzukii Medea Construct

Depositor

Omar Akbari

Article

Buchman et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Inserts

GFP
dsRed
HR5Ie1

Expression

Insect

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pKSB- 3xP3-GFPsv40 GGGG AAGA

Plasmid

#174531

Purpose

3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes

Depositor

Eric Marois

Insert

3xP3-GFP BsaI cassette

Expression

Insect

Promoter

3xP3

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati

Plasmid

#91800

Purpose

Expresses the Drosophila dati circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

dati (dati Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDam-Myc-HP1

Plasmid

#59221

Purpose

expresses Dam-myc-HP1

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Insert

HP1 (Su(var)205 Fly)

Use

Damid

Tags

Dam and myc

Expression

Insect

Promoter

Drosophila heat shock promoter

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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RAT

Plasmid

#187416

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rac1 DN

Plasmid

#52234

Purpose

Drosophila expression of YFP-Rac1 dominant negative mutant

Depositor

Matthew Scott

Insert

Rac1 (Rac1 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T17N; DN mutation

Promoter

GAL4

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pFBXC3GH

Plasmid

#49051

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-SNAP-Csk)

Plasmid

#177895

Purpose

Baculo expression of His10 tag fused to SNAPtag and human Csk

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Csk (CSK Human)

Tags

10xHis-TEV-TVMV-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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HA-GFP_rescue_construct

Plasmid

#190641

Purpose

Puromycin-selectable expression of GFP in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

EGFP

Tags

3xHA

Expression

Insect

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBac[6xP3-Tc-hsp-ECFP-SV40]

Plasmid

#86447

Purpose

pBAc transposon with transformation marker for Tribolium transgenesis; drives ECFP (blue fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid)

Depositor

Gregor Bucher

Insert

6xP3-Tc-hsp-ECFP-SV40

Expression

Insect

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RABIF-FLAG

Plasmid

#135558

Purpose

Express human RABIF in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

RABIF (RABIF Human)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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