5,017 results

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pDW465

Plasmid

#8846

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Tags

biotin acceptor peptide

Expression

Insect

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pF3BGX-TangoL-lexA

Plasmid

#138404

Purpose

RMCE vector for generating C-terminal Tango fusion

Depositor

Shu Kondo

Article

Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

Insert

TEVcs(ENLYFQL)-LexAp65

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBac(3xP3-gTc’v; Tc-hsp68:Cre)

Plasmid

#119063

Purpose

Expression of Cre recombinase in Tribolium castaneum

Depositor

Michalis Averof

Article

Gilles et al Development. 2019 Jan 4;146(1). pii: dev

Inserts

pBacL
Tc-hsp68 Promoter
Cre recombinase
Tc-hsp68 3'UTR
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR

Expression

Insect

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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Dll1ICD-HPC4 pBacPAK8-3

Plasmid

#17333

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Delta-like 1 (Dll1 Mouse)

Tags

HPC4

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac (GST-preSci site-CD45)

Plasmid

#177889

Purpose

Baculo expression of GST tag fused to human CD45 ICD

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

CD45 (aa 598-1304 only) (PTPRC Human)

Tags

TEV-GST-PreScission cut site

Expression

Insect

Mutation

L652P

Promoter

Polyhedrin

Available Since

Aug. 24, 2024

Availability

Academic Institutions and Nonprofits only

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OA-1050E (Ubiq-CasRx)

Plasmid

#132416

Purpose

Express CasRx under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

CasRx

Expression

Insect

Promoter

Ubiq

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab10

Plasmid

#31737

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab10 (Rab10 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP

Plasmid

#221893

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

TAF15 (TAF15 Human)

Tags

6xHis-MBP-PreScission and TEV-mGFP

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH1259-014_Jie_EWSR1_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP

Plasmid

#221894

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

EWSR1 (EWSR1 Human)

Tags

6xHis-MBP-PreScission and TEV-mGFP

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOCC267

Plasmid

#118887

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6-MBP, cleavable with 3C protease; HALO tag

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pUAST mito roGFP2-Grx1

Plasmid

#64995

Purpose

for expression of mitochondrial roGFP2-Grx1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac GST-Plk4

Plasmid

#80264

Purpose

baculovirus creation plasmid for GST-Plk4

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

GST

Expression

Insect

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA mCherry SV40

Plasmid

#176302

Purpose

Expresses mCherry mRNA from the Drosophila Metallothionein A promoter

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

mCherry

Tags

3xFLAG

Expression

Insect

Promoter

MtnA

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 mito roGFP2-Grx1

Plasmid

#65000

Purpose

Expression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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DPYSL4 (DPYSL4A-c003)

Plasmid

#110263

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DPYSL4 (DPYSL4 Human)

Tags

His6-ZBasic-TEV

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBlue-TAL

Plasmid

#49401

Purpose

The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

Depositor

Michal Zurovec

Article

Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

Type

Empty backbone

Use

TALEN

Expression

Insect

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 C450A

Plasmid

#81031

Purpose

Mammalian expression of the dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmARC1_N

Plasmid

#146978

Purpose

Insect Expression of DmARC1

Depositor

Elisa Izaurralde

Insert

DmARC1 (Arc1 Fly)

Expression

Insect

Mutation

5 silent mutations and one non silent mutation G3…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFL-EGFP-3xFlag-Fml1

Plasmid

#193058

Purpose

Baculovirus expression of S.pombe Fml1 for recombinant protein production

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

Fml1 (fml1 Fission Yeast)

Tags

3xFlag

Expression

Insect

Promoter

polyhedron

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST8_FLAGHA_FUS_HIS

Plasmid

#26375

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Tags

FLAG/HA and His

Expression

Insect

Mutation

delta 527 (STOP codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only