4,908 results

1. pMT p85-GFP

   Plasmid

   #126660

   Purpose

   Inducible expression of p85-GFP in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   PI3K SH2 containing subunit
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOCC93

   Plasmid

   #118911

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
   

   Use

   Tags

   eGFP-HIS6

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKM377

   Plasmid

   #134210

   Purpose

   Baculovirus expression of CENP-HKM and His-CENP-I

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   CENP-H/K/M untagged + his-I
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 13XLexAop2 IVS Jaws mCherry_tr

   Plasmid

   #111551

   Purpose

   Optogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry

   Depositor

   Vivek Jayaraman

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJC-TALE-VP64

   Plasmid

   #46147

   Depositor

   David Stern

   Article

   Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

   Type

   Empty backbone

   Use

   Tags

   VP64 activation domain

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUAST tdTomato Rab11 WT

   Plasmid

   #53473

   Depositor

   Matthew Scott

   Insert

   Rab11 (Rab11 Fly)
   

   Use

   Tags

   tdtomato

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAc5.1B-lambdaN-HA-DmStaufen_B

   Plasmid

   #145947

   Purpose

   Insect Expression of DmStaufen

   Depositor

   Elisa Izaurralde

   Article

   Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

   Insert

   DmStaufen (stau Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPTluc

   Plasmid

   #87789

   Purpose

   Luciferase reporter for testing enhancer fragments in Drosophila

   Depositor

   Herman Wijnen

   Article

   Sharp et al Nucleic Acids Res. 2017 Apr 12. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   luciferase

   Expression

   Insect

   Mutation

   Promoter

   minimal promoter

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 1161F

   Plasmid

   #183138

   Purpose

   Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

   Depositor

   Omar Akbari

   Article

   Kandul et al GEN Biotechnology (2022) 1:4, 372-385

   Insert

   U6.3-gRNAs[sxl, bTub]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXN-attPGal4LWL

    Plasmid

    #26261

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUAST mito roGFP2-Orp1

    Plasmid

    #64997

    Purpose

    for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac HT JS-Munc18b

    Plasmid

    #135554

    Purpose

    Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18b (Stxbp2 Rat)
    

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pattB-nsyb-MKII::GAL4DBDo

    Plasmid

    #64721

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    MKIIK11A::GAL4DBDo
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1

    Plasmid

    #192471

    Purpose

    Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS12
    

    Use

    Tags

    AcGFP1

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACEBac1-GCP2

    Plasmid

    #178068

    Purpose

    Insect cell expression of GCP2

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    GCP2 (TUBGCP2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUASTLOTattB_mCherry-CD8-vhhGFP

    Plasmid

    #163930

    Purpose

    Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1025

    Plasmid

    #160235

    Purpose

    FlightN-TetR(OFF)-VP16-Sv40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Tetracycline repressor
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKF297

    Plasmid

    #74774

    Purpose

    template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    TEV- 2xV5 tag (short version)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAcGP67A human LRP6 E3E4 (630–1244)

    Plasmid

    #132628

    Purpose

    expresses human LRP6 E3E4 (630–1244) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (630–1244)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAcGP67A human LRP6 E1E4 (20–1244)

    Plasmid

    #132629

    Purpose

    expresses human LRP6 E1E4 (20–1244) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (20–1244)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge