78,292 results

1. plenti-As-Cas12a-2xNLS

   Plasmid

   #155047

   Purpose

   Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   AsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS, Myc-tag and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti CMV/TO Hygro empty (w214-1)

   Plasmid

   #17484

   Purpose

   3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Venus-173-N-ORAI1

   Plasmid

   #87618

   Purpose

   N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-mKOR

   Plasmid

   #179285

   Purpose

   Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

   Depositor

   Christian Gruber

   Article

   Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

   Insert

   kappa opioid receptor (Oprk1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF hPGK mc2 IRES blast

   Plasmid

   #206239

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-TLR1-YFP

   Plasmid

   #13014

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 1 (TLR1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-D50 lamin A

   Plasmid

   #17653

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

   Insert

   D50 lamin A (LMNA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta 50 (50 aa internal deletion in globular tai…

   Promoter

   CMV

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE-Tau-nYFP

   Plasmid

   #92204

   Purpose

   Retroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   MAPT (MAPT Human)
   

   Use

   Retroviral

   Tags

   YFP(1-155)

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHaloTag-EGFP

   Plasmid

   #86629

   Purpose

   Expresses HaloTag-eGFP tandem (cytosolic)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   HaloTag
   

   Expression

   Mammalian

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LSB-hsa-miR-30d-5p

    Plasmid

    #103417

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-30d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-30d-5p target (MIR30D Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pInducer-FRA1

    Plasmid

    #192267

    Purpose

    Doxycycline-inducible expression of murine FRA1 (Fosl1)

    Depositor

    Günter Schneider

    Article

    Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

    Insert

    Fosl1 (Fosl1 Mouse)
    

    Use

    Lentiviral; Inducible expression

    Expression

    Mammalian

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-PILRA-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156577

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PILRA (PIGR Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-RFP-IKZF1-sh3

    Plasmid

    #69042

    Purpose

    Lentiviral expression of IKZF1 shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+) HIPK3 Sense

    Plasmid

    #60634

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Homeodomain-interacting protein kinase 3 (HIPK3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. mRuby-Clathrin-15

    Plasmid

    #55852

    Purpose

    Localization: Clathrin Vesicles, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Insert

    Clathrin (CLTB Human)
    

    Tags

    mRuby

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pD649-HAsp-PILRA-COMP5AP-AviTag-9xHis

    Plasmid

    #157142

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PILRA (PILRA Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_BE4max_P2A_GFP

    Plasmid

    #112099

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max_P2A_GFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRX-IB-HaloTag7-mGFP-KDEL

    Plasmid

    #184904

    Purpose

    Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    KDEL
    

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF hPGK mc2 IRES hygro

    Plasmid

    #206238

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEM705 Gsz

    Plasmid

    #109355

    Purpose

    GsX chimera with Gαz C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsz chimera
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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