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WT-eNOS-RFP

Plasmid

#22497

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

WT eNOS

Tags

RFP

Expression

Mammalian

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc3-NEDD8

Plasmid

#19943

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)

Tags

myc3

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pEX-CMV-SP-YFP-STM2(15-746)

Plasmid

#18862

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 2 (STIM2 Human)

Tags

YFP

Expression

Mammalian

Mutation

YFP inserted between signal peptide(SP) of STIM1 …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-UTY-F

Plasmid

#17439

Purpose

Expression of UTY in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTY (UTY Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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DAGR

Plasmid

#14865

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

DAG reporter (Prkcb Rat)

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR10-YFP

Plasmid

#13643

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 10 (TLR10 Human)

Tags

YFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-GATA2

Plasmid

#1287

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
Syntaxin4-myc-myc-His

Plasmid

#12377

Depositor

Thomas Weimbs

Article

Low et al Mol Biol Cell. 2006 Feb . 17(2):977-89.

Insert

Syntaxin4 (STX4 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Cloned in modified PCDNA4 vector (double myc)

Promoter

CMV⁄TO

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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p4458 FLAG-hPLIC-1

Plasmid

#8663

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-1 (UBQLN1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pLCK-mScarlet3_C1

Plasmid

#189771

Purpose

Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

LCK-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.1.529

Plasmid

#179907

Purpose

Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

Insert

SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hLRP1-pEZT-BM

Plasmid

#177985

Purpose

Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

Depositor

Lora Hooper

Article

Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

Insert

LDL receptor related protein 1 (LRP1 Human)

Tags

No

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3K(1-10)

Plasmid

#157993

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G27D, D42E, M128I, K153M, T170I

Promoter

SFFV

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pmiRFP680-N1

Plasmid

#136557

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP680

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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hSynapsin-PE2-Cterminal-lenti

Plasmid

#135956

Purpose

Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) C-terminal

Use

Lentiviral

Expression

Mammalian

Mutation

MMLV RT(D200N, T306K, W313F, T330P, L603W)

Promoter

hSynapsin

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV/L7-6-GFP-WPRE

Plasmid

#126462

Purpose

An AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.

Depositor

Hirokazu Hirai

Article

Nitta et al Mol Ther Methods Clin Dev. 2017 Jul 27;6

Insert

L7-6, Purkinje cell specific promoter

Use

AAV

Expression

Mammalian

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PA-Cre

Plasmid

#122960

Purpose

Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_PA-Cre

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.GCaMP6s.WPRE.SV40

Plasmid

#100843

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Ezh2[FL]-dCas9

Plasmid

#100086

Purpose

Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

14056 (Ezh2 Mouse)

Use

CRISPR

Tags

3XFLag-NLS-Ezh2[FL]-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ179

Plasmid

#85996

Purpose

One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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