77,982 results

1. CHFR-PBZ-eGFP

   Plasmid

   #211581

   Purpose

   Expressing CHFR PBZ domain fused to eGFP

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   CHFR PBZ domain (CHFR Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   PBZ domain of the protein

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-OSF-ATG13 (2-197)

   Plasmid

   #188075

   Purpose

   To make ATG101/ATG13 HORMA complex for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)

   Promoter

   CMV

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. RV-mCD4-HH5-4

   Plasmid

   #124984

   Purpose

   Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

   Depositor

   Dan Littman

   Article

   Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

   Insert

   HH5-4 TCRa P2A TCRb
   

   Use

   retroviral

   Expression

   Mammalian

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

   Plasmid

   #234951

   Purpose

   Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

   Plasmid

   #134868

   Purpose

   Expresses EGFP-tagged lysosomal marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER20_mGFP

   Plasmid

   #190027

   Purpose

   Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP

   Depositor

   Andrew Tutt

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ultra-Exon1Q145-Myc-A

   Plasmid

   #110487

   Purpose

   Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)

   Depositor

   Baoji Xu

   Article

   Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

   Insert

   Homo sapiens huntingtin (HTT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Partial long 3'UTR wild type huntingtin exon…

   Promoter

   UbC

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRUPATH Triple Ga15

   Plasmid

   #196060

   Purpose

   Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha15-RLuc8 (GNA15 Human)
   GGamma13-GFP2 (GNG13 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-VSVG

   Plasmid

   #1733

   Purpose

   VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab

   Depositor

   Garry Nolan

   Insert

   VSV-G Envelope
   

   Expression

   Mammalian

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP_WT-talin1 (1-2541)

    Plasmid

    #166112

    Purpose

    For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    WT-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. EXT1-pLJC2-3XFLAG

    Plasmid

    #215818

    Purpose

    Ectopic expression of EXT-1 in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB2A

    Plasmid

    #195283

    Purpose

    Bicistronic DTR and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Hygro (HBEGF Human, Synthetic)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABEpuro-ERBB2 A775_G776insYVMA

    Plasmid

    #40982

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    A775_G776insYVMA

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-2xFLAG-SREBP-1a

    Plasmid

    #26801

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1a (SREBF1 Human)
    

    Tags

    2xFLAG

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. m168

    Plasmid

    #34886

    Depositor

    Irvin Chen

    Article

    Morizono et al Nat Med. 2005 Mar;11(3):346-52. Epub 200

    Insert

    Sindbis virus envelope protein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBbi 41BBL-CD86 mIL-15

    Plasmid

    #183245

    Purpose

    Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-rab7 WT

    Plasmid

    #12605

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-TORCAR-NES

    Plasmid

    #175015

    Purpose

    Cytosol-targeted TORCAR.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    TORCAR-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. TOP-GFP

    Plasmid

    #35489

    Depositor

    Ramesh Shivdasani

    Article

    Horst et al Cancer Res. 2012 Feb 8.

    Insert

    7xTOP-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    7xTCF/LEF

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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