77,958 results

1. qTAG-C-mStayGold-EFS-Puro

   Plasmid

   #227256

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2408

   Plasmid

   #143370

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXF1 (FOXF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-BEACON2

   Plasmid

   #171698

   Purpose

   Expresses BEACON2 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

   Insert

   BEACON2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_W98Y/W104A/Y130F

   Promoter

   CMV

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-ABCB1_STOP

   Plasmid

   #221424

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

   Insert

   ABCB1 (ABCB1 Human)
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV4-p105

   Plasmid

   #23288

   Depositor

   Warner Greene

   Article

   Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

   Insert

   p105 (NFKB1 Human)
   

   Expression

   Mammalian

   Available Since

   April 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. mRuby2-N1

   Plasmid

   #54614

   Purpose

   Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pU6-sgRNA Ef1alpha Puro-T2A-GFP

   Plasmid

   #111596

   Purpose

   sgRNA Ef1alpha Puro-T2A-GFP

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM5-WT PHGDH

   Plasmid

   #83901

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.3_d2eGFP

   Plasmid

   #26821

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-d2eGFP-3'UTR
   

   Expression

   Mammalian

   Mutation

   destabilized (PEST sequence) GFP

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLEX_305

    Plasmid

    #41390

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. VRQR-ABEmax

    Plasmid

    #119811

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. eGFP-luciferase-C1

    Plasmid

    #163523

    Purpose

    Expresses both eGFP and firefly luciferase in mammalian cells

    Depositor

    Thomas Weber

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    eGFP

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pInducer20-Blast

    Plasmid

    #109334

    Purpose

    pInducer20 with added blasticidin resistance

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    Lentiviral; Inducible expression

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-HA_N-human Prkd2

    Plasmid

    #137850

    Purpose

    Express Prkd2 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

    Insert

    Protein kinase d2 (PRKD2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT-TODestFLAGHA_HuB

    Plasmid

    #65755

    Purpose

    expresses FLAGHA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS SK mCherryROSAegfp

    Plasmid

    #54322

    Purpose

    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and EGFP
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIPZ shcGAS-Hsa

    Plasmid

    #128175

    Purpose

    Doxycyclin inducible shRNA knockdown of human cGAS gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shcGAS Mmu (CGAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. LRP6-pCS2

    Plasmid

    #27242

    Depositor

    Xi He

    Article

    Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Expression

    Mammalian

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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