78,002 results

1. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

   Plasmid

   #107943

   Purpose

   Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
   

   Tags

   3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVE-eGFP

   Plasmid

   #52581

   Purpose

   Lentiviral transfer vector driving expression of eGFP in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ExRai-AktAR2

   Plasmid

   #184047

   Purpose

   Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   ExRai-AktAR2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFPC1-EPSIN 2

   Plasmid

   #22242

   Depositor

   Pietro De Camilli

   Article

   Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

   Insert

   Epsin 2 (EPN2 Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

5. GFP-JIP3

   Plasmid

   #164624

   Purpose

   Expresses JIP3 with a GFP tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   JIP3 (MAPK8IP3 Human)
   

   Tags

   cycle 3 GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1_Zeo_TetO_FLAG-AT1R

   Plasmid

   #222856

   Purpose

   Mammalian expression plasmid for FLAG-tagged AT1R

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT1R (AGTR1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pF4Ag NanoLuc (ATG-42)

   Plasmid

   #137777

   Purpose

   Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

   Depositor

   Lance Encell

   Article

   Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

   Insert

   NanoLuc (Nluc)
   

   Expression

   Bacterial and Mammalian

   Mutation

   NanoLuc contains 16 amino acid changes compared t…

   Promoter

   T7 and CMV

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

   Plasmid

   #52421

   Purpose

   Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

   Depositor

   Bertrand Collet

   Insert

   Fusion NLS-EGFP-P2A-Cherry-CAAX
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIV-iRFP720-E2A-Luc

   Plasmid

   #104587

   Purpose

   Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

   Depositor

   Antonius Plagge

   Article

   Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

   Insert

   iRFP720-E2A-Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   bi-cistronic

   Expression

   Mammalian

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Lyso-ExRai-AMPKAR

    Plasmid

    #192449

    Purpose

    ExRai-AMPKAR targeted to the lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Lyso-ExRai-AMPKAR
    

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-hSynI-rtTAV16

    Plasmid

    #102367

    Purpose

    AAV vector to drive TET-ON activator expression from human synapsin I promoter

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

    Insert

    rtTAV16
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    rtTA variant reported in Gene Therapy (2006) 13, …

    Promoter

    human synapsin I promoter

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Integrin-Alpha2-N-18

    Plasmid

    #58192

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: N/A, Emission: N/A

    Depositor

    Michael Davidson

    Insert

    Integrin-Alpha2 (ITGA2 Human)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. NRCoC

    Plasmid

    #74300

    Purpose

    Mammalian expression of full-length treacle fused to GFP

    Depositor

    Rita Shiang

    Article

    Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

    Insert

    treacle ribosome biogenesis factor 1 (Tcof1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. XLone-SOX18

    Plasmid

    #163822

    Purpose

    expresses human SOX18 upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    SOX18 (SOX18 Human)
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 VDAC1-TC

    Plasmid

    #211733

    Purpose

    Expresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Tags

    Tetracysteine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-FLAG-LKB1

    Plasmid

    #8590

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-IZ-ANXA11-mRuby3

    Plasmid

    #184894

    Purpose

    Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA11 (ANXA11 Human)
    

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    R230C relative to NP_001148.1

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dendra2-H2B-6

    Plasmid

    #57724

    Purpose

    Localization: Nucleus Histones, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. mCherry-OSBP

    Plasmid

    #232871

    Purpose

    Expresses OSBP in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    mCherry-OSBP (OSBP Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBGPa-CMV-GFP-OSBP PH domain

    Plasmid

    #58724

    Purpose

    Expresses GFP-Tagged OSBP PH domain in mammalian cells

    Depositor

    Tim Levine

    Article

    Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

    Insert

    Oxysterol-binding Protein PH domain (OSBP Human)
    

    Tags

    GFP and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only