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pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

Plasmid

#205762

Purpose

Single-color FUCCI4 senor for transitions of all four cell cylce phases

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2031

Plasmid

#142906

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PRDM1 (PRDM1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_iPE-C_P2A_Csy4

Plasmid

#232428

Purpose

Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

iPE-C–P2A–Csy4

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-APOBEC1-YTH

Plasmid

#131636

Purpose

Expresses APOBEC1 fused to the YTH domain

Depositor

Kate Meyer

Article

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1 fused to YTH domain (YTHDF2 Human, Rat)

Tags

HA

Expression

Mammalian

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMRXIP TMEM41B-GFP

Plasmid

#117414

Purpose

Expresses TMEM41B tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

TMEM41B (TMEM41B Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPBbsr-mKO-MK2

Plasmid

#115492

Purpose

Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

mKO-MK2 (MAPKAPK2 Human)

Tags

monomeric Kusabira Orange (mKO)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FEN1

Plasmid

#215129

Purpose

Expresses mCherry-FEN1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

FEN1 (FEN1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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PB_iNEUROD1_P2A_GFP_Puro

Plasmid

#168803

Purpose

PiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

NEUROD1 (NEUROD1 Human, Synthetic)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Puro-moxGFP

Plasmid

#227273

Purpose

AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCiPS

Plasmid

#60011

Purpose

Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.

Depositor

David Russell

Article

Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

Insert

Pol

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTI_TetOn_Hu_BIRC5(WT)

Plasmid

#136348

Purpose

Lentiviral inducible expression (TetOn) of human BIRC5

Depositor

Guillermo de Cárcer

Insert

BIRC5 (BIRC5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-P2A-H2A-tdiRFP (JDW 1402)

Plasmid

#242591

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an H2A-tdiRFP for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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piRFP670-N1

Plasmid

#45457

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

iRFP670

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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HRE-doUnaG

Plasmid

#201713

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-VHL-IRES-GFP

Plasmid

#187641

Purpose

VHL Expression vector with GFP

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

VHL (VHL Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Sumo1

Plasmid

#21154

Purpose

Mammalian expression of human Sumo1 with HA tag

Depositor

Junying Yuan

Article

Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

Insert

pcDNA3-HA-Sumo1 (SUMO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-YFP

Plasmid

#11180

Purpose

Mammalian expression vector for expression of YFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

EYFP from Aequorea victoria

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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MCP-APEX2

Plasmid

#154936

Purpose

NLS-V5-MCP-APEX2

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

NLS-V5-MCP-APEX2

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-EXO1a

Plasmid

#111629

Purpose

Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cells

Depositor

Marco Muzi-Falconi

Article

Sertic et al Proc Natl Acad Sci U S A. 2011 Aug 16;10

Insert

Exonuclease 1a (EXO1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-WT

Plasmid

#64060

Purpose

EGFP fused at the N-terminus of tubulin; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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