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pHPHS800

Plasmid

#228240

Purpose

Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Insert

Cas9

Tags

Hygromycin phosphotransferase

Expression

Mammalian

Promoter

none

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FDIO-COX4-dAPEX2

Plasmid

#117178

Purpose

Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV; Flp/frt

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP48

Plasmid

#22585

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP48 (USP48 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV-beta-catenin-IRES-GFP

Plasmid

#14717

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

beta-catenin (Ctnnb1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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EGFP-E-Syt2

Plasmid

#66831

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt2 (ESYT2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-NLS

Plasmid

#199663

Purpose

expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

NLS and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-H2BC11

Plasmid

#207755

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMO14

Plasmid

#235736

Purpose

Expression of human VPS34 complex II in mammalian cells

Depositor

Roger Williams

Article

Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:

Inserts

UVRAG (UVRAG Human)
EGFP
BECN1 (BECN1 Human)
PIK3C3 (PIK3C3 Human)
PIK3R4 (PIK3R4 Human)

Tags

2xStrep-tagII-2xTEV-ZZ

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem

Plasmid

#229792

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pXR004: CasRx pre-gRNA cloning backbone

Plasmid

#109054

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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FAT FAK biosensor

Plasmid

#78303

Purpose

FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

Depositor

Yingxiao Wang

Article

Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

Insert

FAT FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only
pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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GPR123-Tango

Plasmid

#66311

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR123 (ADGRA1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-mitoLbNOX-Flag

Plasmid

#234985

Purpose

Expresses mitochondrial LbNOX in human cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

mito-LbNOX

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCbs FlagOmomyc

Plasmid

#113168

Purpose

Expression of FLAG tagged Omomyc in mammalian cells

Depositor

Sergio Nasi

Article

Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

Insert

Omomyc (MYC Human)

Tags

FLAG

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2WT

Plasmid

#22613

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 WT (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pSLQ10704

Plasmid

#183957

Purpose

U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

SKSKSO array

Use

Lentiviral

Tags

EF1a-Puro-T2A-TagBFP

Expression

Mammalian

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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MTNR1B-DuET

Plasmid

#213349

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

MTNR1B (MTNR1B Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCaggs-mGold

Plasmid

#157996

Purpose

Expression of mGold in mammalian cells

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold

Expression

Mammalian

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

Actin promoter with CMV enhancer

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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M3-PSCFP2

Plasmid

#114185

Purpose

studying clustering of M3 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Article

Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

Insert

Muscarin acetylcholine receptor 3 (CHRM3 Human)

Tags

3xHA and PS-CFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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