78,025 results

1. pBMN mCherry-OPTN(D474N)

   Plasmid

   #119683

   Purpose

   For mammalian expression of UBD mutant OPTN

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D474N

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.2-hBIN1-V5

   Plasmid

   #27303

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Insert

   bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pLIX403-hNOS2

   Plasmid

   #110800

   Purpose

   tet-inducible NOS2 expression

   Depositor

   Edward Morgan

   Article

   Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

   Insert

   NOS2 (NOS2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. xABEmax

   Plasmid

   #119813

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-GG-gRNA

   Plasmid

   #226860

   Purpose

   Human gRNA expression vector backbone with BsmBI Golden Gate site

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-L1PA1CH_blast rescue

   Plasmid

   #69611

   Purpose

   Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

   Depositor

   Astrid Roy-Engel

   Insert

   L1PA1
   

   Tags

   blast rescue cassette

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lck-mTurquoise2

   Plasmid

   #98822

   Purpose

   In vivo visualization of the plasma membrane (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Lck
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP‐RNASEH2A

   Plasmid

   #108700

   Purpose

   For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   RNASEH2A (RNASEH2A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/cyto-iAspSnFR

   Plasmid

   #201394

   Purpose

   CMV overexpression of cyto-iAspSnFR in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

   Insert

   cyto-iAspSnFR
   

   Expression

   Mammalian

   Mutation

   gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

   Promoter

   CMV

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRY2PHR-mCherryN1

    Plasmid

    #26866

    Purpose

    Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2PHR (CRY2 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHRm-NLS-dCas9-GFP11x7-NLS

    Plasmid

    #70224

    Purpose

    Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    NLS-dCas9-GFP11x7-NLS
    

    Tags

    GFP11x7

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-neo-PP1-Alpha

    Plasmid

    #171267

    Purpose

    Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-PGK-EGFP-ALDH1A2-Neo

    Plasmid

    #189743

    Purpose

    The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A2 (ALDH1A2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PE-Plus

    Plasmid

    #214079

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PEA1-Puro

    Plasmid

    #171991

    Purpose

    Delivers all prime editing nickase components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. FKBP-V5-AP-nes_pLX304

    Plasmid

    #120912

    Purpose

    Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    FKBP-V5-AP-NES
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Cyto-GCaMP3

    Plasmid

    #64853

    Purpose

    Cytosol-targeted version of the Ca2+ indicator GCaMP3.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    GCaMP3
    

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-puro-myr-HA-Akt1

    Plasmid

    #46969

    Purpose

    lentiviral expression of myr-HA-Akt1

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    HA and myr

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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