81,859 results
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pcDNA-R-iLACCO1.2
Plasmid#208027PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1.2
ExpressionMammalianAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_IRES_GFP_CD16a
Plasmid#196189PurposeRetroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.DepositorInsertCD16a (FCGR3A Human)
UseRetroviralExpressionMammalianMutationchanged Phenylalanine 158 to ValinePromoterLTRAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.19_PB-CIT-PGK-Blast
Plasmid#205564PurposeExpress human CITDepositorAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
psPax2-D64V-NC-mNeon
Plasmid#196509PurposemNeon fluorescent protein fused to nucleocapsid protein in psPAX2DepositorInsertmNeon
TagsmNeonExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEd-V106W
Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
ExpressionMammalianAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
SspBmicro-mCh-p60
Plasmid#190168PurposeExpresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.DepositorInsertSspBmicro-mCherry-p60
TagsSspBmicro-mCherryExpressionMammalianAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/er-(n2)oxStayGold(c4)
Plasmid#185822PurposeA soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.DepositorInserter-(n2)oxStayGold(c4)
ExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)
Plasmid#185910PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7Available SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR2
Plasmid#184047PurposeImproved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.DepositorInsertExRai-AktAR2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
GalT-RpHLuorin2
Plasmid#171719PurposeTrans-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 B.1.617.2v1
Plasmid#179905PurposeEncodes SARS-CoV-2 B.1.617.2v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianMutationT19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…PromoterCAGAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Luc-noPS
Plasmid#177940PurposeTransient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorInsertLuc2
ExpressionMammalianAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon 300
Plasmid#163115PurposeRed fluorescent protein-based glucose sensor for mammalian cells with higer affinityDepositorInsertRed Glifon 300
ExpressionMammalianPromoterCMVAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT001
Plasmid#171061PurposePlasmid for expressing HIV-1 envelope glycoproteinDepositorInsertHIV-1 Env
UseLentiviralExpressionMammalianPromoterCAGAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-mTurquoise2-IL-1beta-mNeonGreen
Plasmid#166783PurposeFRET analysis (pro-IL-1beta cleavage) in living cellsDepositorInsertmTurquoise2-IL-1b-mNeongreen (IL1B Human)
UseLentiviralTagsmNeonGreen and mTurquoise2ExpressionMammalianPromoterCMVAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cas9-2A-BFP
Plasmid#164662PurposeRetroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent proteinDepositorInsertsCas9
BFP
UseCRISPR and RetroviralExpressionMammalianPromoterMSSV_LTRAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mGreenLantern
Plasmid#164469PurposeConstitutive CAG-driven expression of mGreenLantern in animals using AAVDepositorInsertmGreenLantern
UseAAVExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCAGAvailable SinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term His8 eGFP 3C
Plasmid#160684PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTagsHis8 eGFP 3CExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC1-mitoRexYFP
Plasmid#60246PurposeMitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.DepositorInsertmitoRexYFP
TagsMitochondrial targeting signalExpressionMammalianPromoterCMVAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only
