78,043 results

1. PMmEos3.2

   Plasmid

   #203755

   Purpose

   Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Lyn n-term (LYN Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOS005: superecliptic pHluorin (cytosolic)

   Plasmid

   #163049

   Purpose

   superecliptic pHluorin (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   superecliptic pHluorin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human USP7-3X FLAG

   Plasmid

   #225334

   Purpose

   Lentiviral expression of human USP7 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 (USP7 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCMV-SS-antiFLAGscFv-TM

   Plasmid

   #198303

   Purpose

   Displays anti-FLAG scFv from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiFLAGscFv-TM
   

   Expression

   Mammalian

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVTNT-T7-KPNA4

   Plasmid

   #26680

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 4 (KPNA4 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas3-Amp

   Plasmid

   #207998

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-VP40

   Plasmid

   #74421

   Purpose

   Mammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusion

   Depositor

   Judith White

   Article

   Shoemaker et al PLoS One. 2013;8(2):e56265. doi: 10.1371

   Insert

   VP40
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. opto-E-cad GFP

   Plasmid

   #203327

   Purpose

   Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

   Depositor

   Seraphine Wegner

   Article

   Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

   Insert

   Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Addition of Aslov2 domain at T133 of human E-cadh…

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBR_attB(bxb)_lox

   Plasmid

   #183762

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads <20 kb

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-PIK3CG

    Plasmid

    #174189

    Purpose

    Expresses PIK3CG in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

    Insert

    PIK3CG (PIK3CG Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid

    #71657

    Purpose

    mammalian expression of eiF4A1

    Depositor

    Gideon Dreyfuss

    Insert

    eiF4A1 (EIF4A1 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    aa403 from Ala to Arg

    Promoter

    CMV

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-FLIM-AKAR

    Plasmid

    #63058

    Purpose

    Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKAR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. p4xSTAT6-Luc2P

    Plasmid

    #35554

    Depositor

    Axel Nohturfft

    Insert

    4xStat6 promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    4xStat6

    Available Since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPR158-Tango

    Plasmid

    #66332

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR158 (GPR158 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pLenti6/V5-DEST-SPAG5

    Plasmid

    #31217

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    SPAG5 (SPAG5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Contains a one nucleotide deletion at C-term that…

    Available Since

    Aug. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)

    Plasmid

    #163043

    Purpose

    QuasAr2-mOrange2 voltage sensor (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    QuasAr2
    

    Use

    Lentiviral

    Tags

    mOrange2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-mDlx-ChR2-mCherry-Fishell-3

    Plasmid

    #83898

    Purpose

    ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    ChR2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. PKC zeta DN

    Plasmid

    #21249

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC zeta (Prkcz Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K281M

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pHPHS800

    Plasmid

    #228240

    Purpose

    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

    Insert

    Cas9
    

    Tags

    Hygromycin phosphotransferase

    Expression

    Mammalian

    Promoter

    none

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMEH380 VSV-G HA

    Plasmid

    #168222

    Purpose

    Mammalian expression of vesicular stomatitis virus glycoprotein fused to a C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    VSV-G
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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