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AAVS1-PuroR Xlone eGFP

Plasmid

#179837

Purpose

Doxycycline-inducible expression of eGFP

Depositor

Xiaoping Bao

Article

Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN ; Donor plas…

Expression

Mammalian

Promoter

TRE3GS

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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WT GLUT1-GFP

Plasmid

#200093

Purpose

WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDsRed-Max-N1

Plasmid

#21718

Depositor

Benjamin Glick

Article

Strack et al Nat Methods. 2008 Nov . 5(11):955-7.

Insert

DsRed-Max

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-TagRFP657

Plasmid

#96939

Purpose

Retroviral plasmid containing TagRFP657 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Tag Red Fluorescent Protein 657

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

Plasmid

#236422

Purpose

transient overexpression of KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

KEAP1 (KEAP1 Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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SH4-FRB-EBFP2

Plasmid

#222418

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

SH4

Tags

FRB-HA-EBFP2

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-RoninDHSA-FLAG-IRES-Neo

Plasmid

#28021

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Tags

FLAG

Expression

Mammalian

Mutation

DHSA mutation (Y246A) of the previously defined H…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-CGBE1

Plasmid

#198537

Purpose

Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-CGBE1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL hygro Flag HA TRAP220 wt

Plasmid

#17433

Purpose

Retroviral expression of Med1/TRAP220 in mammalian cells

Depositor

Kai Ge

Article

Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

Insert

TRAP220 (MED1 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA4

Plasmid

#26680

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 4 (KPNA4 Human)

Tags

T7

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a-QL

Plasmid

#86076

Purpose

A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed glutamine 67 to leucine

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA6 asyn WT

Plasmid

#107425

Purpose

Mammalian expression of human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein (SNCA Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV4-L

Plasmid

#74982

Purpose

Constitutive ETV4 ORF, blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 Human)

Expression

Mammalian

Mutation

S396N

Promoter

EF1a

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-p18G2A-HA

Plasmid

#42327

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 G2A (LAMTOR1 Human)

Tags

HA

Expression

Mammalian

Mutation

G2A

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pLPCX roGFP2-Orp1

Plasmid

#64991

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-APOBEC1-YTHmut

Plasmid

#131637

Purpose

Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

Depositor

Kate Meyer

Article

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1-YTHmut-HA (YTHDF2 Rat, Human)

Tags

HA

Expression

Mammalian

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC4A8

Plasmid

#132300

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A8 (SLC4A8 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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PB-SAM

Plasmid

#102559

Purpose

Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Inserts

MS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Mutation

D10A and N863A in Cas9 and N55K in MS2

Promoter

CAG and EF1A

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMarsL274G

Plasmid

#63177

Purpose

This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

Depositor

David Tirrell

Article

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Insert

MarsL274G (Mars1 Mouse)

Expression

Mammalian

Mutation

L274G

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-SET-FLAG-HA

Plasmid

#24998

Depositor

Judy Lieberman

Article

Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Insert

SET (SET Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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