78,128 results

1. pcDNA-ET-I654Q

   Plasmid

   #72545

   Purpose

   expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   BRD4 ET (BRD4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   I654Q

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-ET-L630W

   Plasmid

   #72543

   Purpose

   expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   BRD4 ET (BRD4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L630W

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIP-SFFV-mtagBFP-2A STING

   Plasmid

   #102586

   Purpose

   Lentivector to express STING and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MTS-mCherry-GFP1-10-Hyg-N1

   Plasmid

   #91957

   Purpose

   Expresses mCherry-GFP1-10 in mammalian cell mitochondria

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   MTS-mCherry-GFP1-10
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-tetO-NGN2

   Plasmid

   #197090

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2 (NEUROG2 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF3290

   Plasmid

   #144766

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CTCF (CTCF Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mRFP-Ub

   Plasmid

   #11935

   Depositor

   Nico Dantuma

   Article

   Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

   Insert

   Ubiquitin (RBX1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

   Plasmid

   #216154

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic exon and C-terminal FLAG
   

   Expression

   Mammalian

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

   Plasmid

   #198863

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human TERT gene

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   TERT sgRNA (TERT Human)
   

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211462

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS_3E5E_luciferase

    Plasmid

    #103055

    Purpose

    Encodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    Ebolavirus minigenome
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. EMTB-mCherry

    Plasmid

    #26742

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    2x mCherry and Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCAG EGFP

    Plasmid

    #14857

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2002 Feb 19. 9

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 IKKE K38A flag

    Plasmid

    #26202

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon K38A (IKBKE Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Catalytic Lysine changed to Alanine (K38A)

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SMVP-Cas9N

    Plasmid

    #80930

    Purpose

    Expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-teLuc c-myc

    Plasmid

    #100026

    Purpose

    Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    teLuc, a NanoLuc mutant
    

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    D19S, D85N, C164H

    Promoter

    CMV

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-mPCSK7

    Plasmid

    #122676

    Purpose

    Expresses mouse PCSK7 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-hApg5

    Plasmid

    #22952

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

    Insert

    Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK486 (Hygro-P2A-2mAID)

    Plasmid

    #214398

    Purpose

    2mAID for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-2mAID
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. VEGFR2-mCh

    Plasmid

    #108854

    Purpose

    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

    Insert

    KDR (KDR Human)
    

    Tags

    Labeled with mCherry on the C-Terminus via a 3 am…

    Expression

    Mammalian

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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