79,308 results

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B52 (empty plasmid backbone to express 2 sgRNAs)

Plasmid

#100708

Purpose

Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF2541

Plasmid

#142399

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX5 (SOX5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Oct4-EGFP

Plasmid

#21319

Purpose

Lentiviral vector, expresses EGFP under Oct3/4 promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Oct-4 promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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PX459v3

Plasmid

#178799

Purpose

SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-hTBK1-EGFP

Plasmid

#221553

Purpose

Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-Trono-EFS-Blast-P2A-TurboRFP

Plasmid

#228893

Purpose

Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-E1799K

Plasmid

#69009

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-E1799K (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Glutamate 1799 to Lysine

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-BAF

Plasmid

#227442

Purpose

Mammalian expression of GFP-BAF

Depositor

David Pellman

Article

Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

Insert

Barrier-to-autointegration factor (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSN837

Plasmid

#184825

Purpose

Expresses human KIF5A in mammalian cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (KIF5A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-pcDNA3.1 Puro-CAG-Positron2

Plasmid

#239079

Purpose

Mammalian expression of positive-going voltage sensor

Depositor

GENIE Project

Article

Eric R. et al Janelia Figshare 2023

Insert

Positron2

Expression

Mammalian

Mutation

R78K N81D D92N W178F

Promoter

CAG

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNG-IRES-mScarlet-dMCP

Plasmid

#231089

Purpose

Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

mNG-IRES-mScarlet-dMCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX

Plasmid

#208692

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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1227 pcDNA3 Myr HA Akt2

Plasmid

#9016

Depositor

William Sellers

Insert

Akt2 (AKT2 Human)

Tags

HA and Myr

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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FLAG-3C-sGFP11_pcDNA3.1(-)

Plasmid

#182932

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Tags

3C-cleavable split GFP 11, FLAG tag, and HA signa…

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

Plasmid

#190162

Purpose

Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

Depositor

Samie Jaffrey

Article

Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

Insert

mcherry_(3'UTR, No Actin)_24Broccoli

Tags

24×Broccoli

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-HA-OTUD7B

Plasmid

#22550

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUD7B (OTUD7B Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b-gRNA non target

Plasmid

#223698

Purpose

gRNA control for dPspCas13b-FTO

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

gRNA target sequence for luciferase control

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

Plasmid

#185916

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8.20m-nSpG-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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DsRed-rab7 WT

Plasmid

#12661

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(PGK/CMV)-12xHRE

Plasmid

#172335

Purpose

hypoxic responsive reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

PGK/CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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