75,820 results

1. Double UP mNeon to mScarlet Rac1-V12

   Plasmid

   #125136

   Purpose

   Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).

   Depositor

   Erik Dent

   Article

   Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

   Insert

   mNeon/mScarletP2A Rac1-V12
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Removed Not1 site in mScarlet. 12th AA in Rac1 ch…

   Promoter

   CAG

   Available since

   May 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP

   Plasmid

   #32476

   Purpose

   Expresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAM Y115F,L141F-5HT3 HC
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. Spectrin-C-cpstFRET

   Plasmid

   #61111

   Purpose

   Expression of Spectrin-C-term cpstFRET to measure mechanical stress and report multidimensional stress fields

   Depositor

   Fred Sachs

   Article

   Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

   Insert

   spectrin-cpstFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDG461

   Plasmid

   #100902

   Purpose

   SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1a154 (H77)

   Plasmid

   #177689

   Purpose

   HCV E1E2 expression plasmid

   Depositor

   Justin Bailey

   Article

   Salas et al Gastroenterology. 2022 Feb;162(2):562-57

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-SasgRNA scaffold-CMV-nSaCas9

   Plasmid

   #171695

   Purpose

   Expresses nSaCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   sgRNA scaffold-nSaCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CMV

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFuse-STOP

   Plasmid

   #217677

   Purpose

   Empty vector control plasmid containing a stop codon prior to the Fc encoding region

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-HA-BACE1

   Plasmid

   #165032

   Purpose

   HA-BACE1

   Depositor

   Juan Bonifacino

   Article

   Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

   Insert

   BACE1 (BACE1 Human)
   

   Use

   Tags

   HA (embedded after propeptide cleavage site)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.Flex.GCaMP6s.WPRE.SV40

   Plasmid

   #100842

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. a1Ha-pcDNA3

    Plasmid

    #45809

    Depositor

    Edward Perez-Reyes

    Article

    Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

    Insert

    a1Ha (CACNA1H Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. AbVec2.0-IGHG2

    Plasmid

    #99576

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG2 isotype

    Depositor

    Hedda Wardemann

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PresentER-SIINFEKL (mCherry)

    Plasmid

    #102945

    Purpose

    PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    SIINFEKL (OVAL Synthetic, Chicken)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    OVA257-264

    Promoter

    LTR

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

13. Flag-FOXF2

    Plasmid

    #153123

    Purpose

    Expression of Flag tagged human FOXF2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXF2 (FOXF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. T7-RelA

    Plasmid

    #21984

    Depositor

    Warner Greene

    Article

    Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

    Insert

    NFkB p65 subunit (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    For more details for this expression construct,…

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

15. dCas9-5xGCN4-P2A-BFP

    Plasmid

    #184438

    Purpose

    dCas9 fused with 5 copies of GCN4 epitope

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    dCas9-5xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRESpuro-Flag-Pol b

    Plasmid

    #23257

    Depositor

    Robert Sobol

    Article

    Tang et al Mol Cancer Res. 2010 Jan 12. ():.

    Insert

    DNA polymerase beta (POLB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

    Plasmid

    #79620

    Purpose

    Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1 and UGI

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-N-Myc_C223S USP7

    Plasmid

    #131243

    Purpose

    Mammalian expression of catalytically inactive Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains a point mutation that converts USP7 cyst…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFPN1-human Dynamin 1aa K44A

    Plasmid

    #22197

    Depositor

    Pietro De Camilli

    Article

    Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

    Insert

    human Dynamin 1aa K44A (DNM1 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. MRGPRD-Tango

    Plasmid

    #66435

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MRGPRD (MRGPRD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only