75,869 results

1. pcDNA4-PKCZeta WT His tagged

   Plasmid

   #24609

   Depositor

   Jeff Wrana

   Article

   Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

   Insert

   PKCZeta (PRKCZ Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pKH3-TRIM28

   Plasmid

   #45569

   Depositor

   Fanxiu Zhu

   Article

   Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. Flag-Gsdmd-NT 4A

   Plasmid

   #80952

   Purpose

   Expresses Gsdmd-NT 4A mutant in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd-NT 4A (Gsdmd Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-GRAM-H

   Plasmid

   #211700

   Purpose

   Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (EGFP-GRAM1b G187L) in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Ercan et al EMBO J. 2021 Mar 15;40(6):e106524. doi:

   Insert

   GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Leucine

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-HcRed

   Plasmid

   #11152

   Purpose

   Mammalian expression vector for expression of HcRed

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Insert

   HcRed1 from Heteractis crispa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLexM-SLC3A2

   Plasmid

   #197994

   Purpose

   Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC3A2 (SLC3A2 Human)
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Please see depositor comments below

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330-SpCas9-NG

   Plasmid

   #117919

   Purpose

   Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pInducer-YAP1-S127A/S397A/S94A

   Plasmid

   #213586

   Purpose

   Doxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cells

   Depositor

   Channing Der

   Article

   Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S127A, S397A, S94A

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST26-C-FLAG

   Plasmid

   #79275

   Purpose

   Destination vector containing a C-terminal FLAG tag

   Depositor

   Rita Shiang

   Article

   Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

   Insert

   FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCGN-HCF-1 fl

    Plasmid

    #53309

    Purpose

    plasmid contains human HCFC1 full length gene with HA and c-myc tags

    Depositor

    Winship Herr

    Article

    Wilson et al Cell. 1993 Jul 16;74(1):115-25.

    Insert

    HCFC1 (HCFC1 Human)
    

    Use

    Tags

    HA and c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti6.2_mCherry_roGFP2_Orp1

    Plasmid

    #155043

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid

    #118162

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-BRIC

    Plasmid

    #172338

    Purpose

    Bioluminescent Red Indicator for Calcium

    Depositor

    Huiwang Ai

    Article

    Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

    Insert

    BRIC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-GFAP-TurboID

    Plasmid

    #227179

    Purpose

    Co-expression of FLAG-tagged-TurboID and T2A-mCherry under the GFAP short promoter by AAV vector

    Depositor

    Junmin Peng

    Article

    Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

    Insert

    TurboID
    

    Use

    AAV

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP_short

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

    Plasmid

    #169337

    Purpose

    Expression of the LT-Fucci(SCA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SCA) (GMNN, CDT1 Human)
    

    Use

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

    Plasmid

    #191146

    Purpose

    Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    Bdnf.t1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-IP GFP-NBR1

    Plasmid

    #38283

    Depositor

    Noboru Mizushima

    Article

    Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

    Insert

    Next to BRCA1 gene 1 protein (NBR1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 3xmScarletI_UtrCH

    Plasmid

    #112962

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    UtrCH
    

    Use

    Tags

    3x mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3-AUP1-FLAG

    Plasmid

    #185336

    Purpose

    Mammalian expression of AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. XLone-ETV2

    Plasmid

    #135763

    Purpose

    expresses human ETV2 upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    ETV2 (ETV2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    TRE3G

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge