78,128 results

1. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42229

   Purpose

   This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSAM2_mCherry_Gata6

   Plasmid

   #72694

   Purpose

   Doxycyline regulated expression of Gata6 in mammalian cells

   Depositor

   Timothy Kamp

   Article

   Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

   Insert

   Gata 6 (Gata6 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV-Doxycycline

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LBR EGFP

   Plasmid

   #128150

   Purpose

   Expression of lamin B receptor

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

   Insert

   lamin B receptor (LBR Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF0346

   Plasmid

   #143397

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MYT1L (MYT1L Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. hGli2 flag3x

   Plasmid

   #84920

   Purpose

   mammalian expression of Gli2

   Depositor

   Martin Fernandez-Zapico

   Insert

   Gli2 (GLI2 Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL3617 (pCRISPR(BbsI)_Pse)

   Plasmid

   #200902

   Purpose

   Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseCRISPR (entry)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ABE8r-V106W

   Plasmid

   #208295

   Purpose

   Expresses ABE8r in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r-V106W-SpCas9 D10A
   

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LeGO-T2

   Plasmid

   #27342

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-SP-FRB (T2098L) -sfGFP-TM-V5

   Plasmid

   #186227

   Purpose

   mRNA synthesis of rapalog-induced cell adhesion molecule

   Depositor

   Yoshihiro Morishita

   Article

   Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

   Insert

   mRNA synthesis of rapalog-induced cell adhesion molecule
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   FRB (T2098L)

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FURIN-bio-His

    Plasmid

    #51755

    Purpose

    Expresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    FURIN (FURIN Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

    Plasmid

    #216694

    Purpose

    Synchronize trafficking of GPI-AP from the ER (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    SBP-mApple-FIREtag-GPI
    

    Tags

    FIREtag, IL2 signal sequence, Streptavidin Bindin…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHRIG-Akt1

    Plasmid

    #53583

    Purpose

    lentiviral expression of constitutively active human Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    cAkt1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available Since

    Oct. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. PKCalpha.FLAG

    Plasmid

    #10805

    Depositor

    Alex Toker

    Article

    Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

    Insert

    PKC alpha (PRKCA Bovine)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D332G-please see comments below

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-Sp-pegRNA-RNF2_+5GtoT

    Plasmid

    #135957

    Purpose

    S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    RNF2_5GtoT pegRNA
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

    Plasmid

    #172627

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLNCX2-mEGFP-TSG101

    Plasmid

    #116925

    Purpose

    expresses mEGFP-TSG101 in mammalian cells

    Depositor

    Sanford Simon

    Article

    Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

    Insert

    TSG101 (TSG101 Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ATR Kinase dead

    Plasmid

    #31612

    Depositor

    Aziz Sancar

    Article

    Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

    Insert

    ATR (ATR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K2327R (kinase dead)

    Available Since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-HA-COPS5

    Plasmid

    #22541

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    COPS5 (COPS5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. EFSp-GFP-Empty

    Plasmid

    #174171

    Purpose

    Control lentiviral vector expressing GFP under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Sec61 beta pAcGFP-C1 (802)

    Plasmid

    #62008

    Purpose

    mammalian expression of GFP-Sec61 beta

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only