78,128 results

1. pENTR-L1-PGK-PuroTK-L2

   Plasmid

   #141005

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   PuroTK
   

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-US-ECasE

   Plasmid

   #83961

   Purpose

   Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

   Depositor

   Eleanor Chen

   Insert

   ERT2-spCas9-ERT2
   

   Expression

   Mammalian

   Mutation

   please see depositor comments below

   Promoter

   U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-StrepII

   Plasmid

   #208617

   Purpose

   Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   Strep II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_U6-ωRNA*_ISDra2-TnpB-mNG

   Plasmid

   #212968

   Purpose

   AAV vector for encoding an ISDra2-TnpB driven by EFs promoter

   Depositor

   Chunlong Xu

   Article

   Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

   Inserts

   ISDra2-TnpB-T2A-mNG,
   ωRNA*
   

   Use

   AAV and CRISPR

   Tags

   NLS and NLS-FLAG

   Expression

   Mammalian

   Promoter

   EF1a, T7 and U6

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR- flag-Lag16-JAM-B

   Plasmid

   #205200

   Purpose

   This vector encodes of the synCAM (JAM-B) and GFP nanobody LAG16

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM JAM-B and Lag16 anti-GFP nanobody
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-ccdB-V5

   Plasmid

   #214985

   Purpose

   Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag

   Depositor

   Wade Harper

   Type

   Empty backbone

   Tags

   V5

   Expression

   Mammalian

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-Akt1(WT)

   Plasmid

   #86631

   Purpose

   Expresses mCherry-tagged human Akt1(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1054

   Plasmid

   #142720

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PLAGL1 (PLAGL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRX1 flag C35S

   Plasmid

   #21285

   Depositor

   Wang Min

   Article

   Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

   Insert

   TRX1 (TXN Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C35S

   Available Since

   July 30, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

    Plasmid

    #141373

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMX-Flag-GFP_HOMER2

    Plasmid

    #227953

    Purpose

    Co-localisation experiment

    Depositor

    Alessandro Ori

    Insert

    HOMER2 (HOMER2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG-EGFP

    Expression

    Mammalian

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF3157

    Plasmid

    #144633

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMARCD1 (SMARCD1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pN3-Nipbl

    Plasmid

    #107716

    Purpose

    Expression plasmid for 3xFLAG-tagged NIPBL

    Depositor

    Guntram Suske

    Insert

    NIPBL (Nipbl Mouse)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGF1-4eCOL2A1

    Plasmid

    #97210

    Purpose

    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

    Depositor

    Ryan Porter

    Article

    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

    Insert

    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    COL2A1 regulatory elements

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-ZIKVA-GFP-puro

    Plasmid

    #140090

    Purpose

    Fluorescence activatable reporter of ZIKV NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    ZIKVA-GFP
    

    Use

    Lentiviral; Low expression

    Tags

    6XHis tag and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBbsr2-4031NES

    Plasmid

    #105241

    Purpose

    A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

    Depositor

    Michiyuki Matsuda

    Article

    Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

    Insert

    AMPK-EV
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBB-XIAP

    Plasmid

    #11558

    Depositor

    Jon Ashwell

    Article

    Yang et al Science 2000 May 5;288(5467):874-7.

    Insert

    XIAP (XIAP Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMEH31 Lamp2b HA

    Plasmid

    #168208

    Purpose

    Mammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. cycLuc_TEVS

    Plasmid

    #119207

    Purpose

    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with TEVs cleavage site
    

    Use

    Luciferase

    Tags

    6xHis and AU1 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHBV-Luc

    Plasmid

    #71414

    Purpose

    To see the activity of HBV transcription using Luciferase assay

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

    Insert

    HBV core promoter, enhancer I, enhancer II and luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    HBV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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