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pRC/CMV-JAK1-VSV

Plasmid

#139356

Purpose

Expression of JAK1 protein

Depositor

Sandra Pellegrini

Publication

Richter et al J Biol Chem. 1998 Sep 18;273(38):24723-9

Insert

JAK1 cDNA with 300nt of 5'UTR (JAK1 Human)

Tags

VSV

Expression

Mammalian

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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sgTrack-mCherry

Plasmid

#114013

Purpose

Empty sgRNA expression vector with co-expression of mCherry reporter

Depositor

Charles Rudin

Publication

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-FLAG-SOX4

Plasmid

#110360

Purpose

Mammalian expression of FLAG-tagged SOX4

Depositor

Carlos Moreno

Publication

Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

Insert

SOX4 (SOX4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lacking 5' UTR

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTfR-mNeonGreen

Plasmid

#129608

Purpose

In vivo visualization of Transferrin receptors (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Transferrin

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A1

Plasmid

#132103

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A1 (SLC2A1 Human)

Expression

Mammalian

Available since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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mARG1 cassette 1

Plasmid

#134343

Purpose

Mammalian acoustic reporter gene 1 cassette 1 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Publication

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 1

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTRIPZ shcGAS-Hsa

Plasmid

#128175

Purpose

Doxycyclin inducible shRNA knockdown of human cGAS gene

Depositor

Sandra Demaria

Publication

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shcGAS Mmu (CGAS Human)

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSLQ-5S-F30-Mango IV

Plasmid

#127584

Purpose

Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

Depositor

David Rueda

Publication

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold and Mango IV tag

Expression

Mammalian

Promoter

modified U6

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Publication

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMGS36 (GFP-ARF16-PB1)

Plasmid

#126581

Purpose

Construct to express GFP-ARF16-PB1 under the control of the CMV promoter

Depositor

Michael Guertin

Publication

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OsARF16-PB1 domain

Tags

GFP

Expression

Mammalian

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

Plasmid

#107700

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Publication

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3-P2A-CheRiff

Use

AAV

Expression

Mammalian

Promoter

human synapsin I

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-LMNA-gRNA1

Plasmid

#122507

Purpose

expresses WT spCas9 and a chimeric gRNA targeting human LMNA

Depositor

Graham Dellaire

Publication

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LMNA-gRNA1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLenti c-MYC-DDK-Puro GFP

Plasmid

#123299

Purpose

Mammalian expression of GFP C-MYC-FLAG

Depositor

Giovanni Tonon

Publication

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

GFP

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

Plasmid

#119945

Purpose

Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

Depositor

Jeffrey Chao

Publication

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Renilla luciferase

Tags

24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

Expression

Bacterial and Mammalian

Promoter

TetCMV

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLenti-CMV-GFP-Sox11

Plasmid

#120387

Purpose

over expression of sox11 using infection

Depositor

Igor Ulitsky

Publication

Perry et al Mol Cell. 2018 Nov 1;72(3):553-567.e5. d

Insert

sox11 (Sox11 Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+)-HA-Flag-natT1R2

Plasmid

#113944

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Publication

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TNFR2-GFP

Plasmid

#111207

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNFRSF1B (TNFRSF1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

S175P, D306G

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMV-NLS-AcrIIA4

Plasmid

#113037

Purpose

Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Publication

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SV40 NLS-AcrIIA4

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGW1-mCherry-EGFP-PIM

Plasmid

#111759

Purpose

High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

Depositor

Lukas Kapitein

Publication

Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

CMV

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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