80,205 results
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Plasmid#192451PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit.DepositorAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only
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pCAG-ChroMD-mRuby2-ST
Plasmid#171158PurposeCation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroMD
ExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGENE B::AtHAP2-V5
Plasmid#132959PurposeExpress AtHAP2 full length protein in mammalian cellsDepositorInsertAtHAP2::V5
TagsV5ExpressionMammalianAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-ZEB2-P2A-mCherry
Plasmid#203857PurposeTet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertZEB2 (ZEB2 Human)
ExpressionMammalianAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Flag-SRm160
Plasmid#11305DepositorAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHR EGFR-CD3ε-FusionRed
Plasmid#188626PurposeHuman EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensorDepositorInsertEGFR (EGFR Human, Synthetic)
UseLentiviralTagsCD3ε-FusionRedExpressionMammalianPromoterSFFVAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p80-Katanin-mNeonGreen
Plasmid#191762PurposeExpression of mNeonGreen fused to p80-KataninDepositorInsertp80-Katanin-mNeonGreen (KATNB1 Human)
TagsmNeonGreenExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianPromoterEF1aAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCR8-DuET
Plasmid#213204PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
iSpyMac
Plasmid#155014PurposeExpresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cellsDepositorInsertiSpyMac
ExpressionMammalianMutationPID grafted onto SpyCas9 N-terminal domain, R221K…PromoterEF1ɑAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV BAF170-Flag
Plasmid#19142DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)
TagsFlagExpressionMammalianAvailable SinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP9A-HA
Plasmid#209213PurposeMammalian expression of ATP9ADepositorAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1a-EGFP
Plasmid#138327Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1a
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianPromotersynapsinAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_070
Plasmid#164265PurposeLentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR, Cre/Lox, and LentiviralExpressionMammalianPromoterHuman U6Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG NUFIP1
Plasmid#110950Purposeexpresses mammalian FLAG-NUFIP1DepositorAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-LMNB1
Plasmid#207771PurposeDonor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorInsertLMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
ClopHensorN
Plasmid#50758PurposeMammalian expression vector expressing the ClopHensorN chloride and proton sensorDepositorInsertClopHensorN
TagsStrep-IIExpressionMammalianMutationE2GFP is EGFP-T203YPromoterCAGAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-mCherry
Plasmid#108122PurposeRecruitable fluorophoreDepositorAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab4B
Plasmid#61801PurposeHuman Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expressionDepositorAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only