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Addgene

4,240 results

Showing: 1361 - 1380 of 4240 results
  1. pSW725 - pUFO>>

    Plasmid
    #116000
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Insert
    pUFO:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Use
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pFGC-I2Cas9

    Plasmid
    #173158
    Purpose
    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.
    Depositor
    Inserts
    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    Use
    Tags
    3xFLAG-NLS and NLS
    Expression
    Plant
    Mutation
    Contains a D169N mutation with no visible effect …
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pGWB401NL3F10H

    Plasmid
    #141285
    Purpose
    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)
    Depositor
    Type
    Empty backbone
    Use
    Luciferase; Gateway
    Tags
    FLAG (x3), HIS (x10), and NLUC
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pFH113

    Plasmid
    #128210
    Purpose
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
    Depositor
    Insert
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pdCas9 (GB1079)

    Plasmid
    #75399
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
    Depositor
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1361 - 1380 of 4240 results