4,552 results

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pCBLsgRNA7

Plasmid

#199733

Purpose

Golden Gate entry vector; 7th gRNA under OsU6.2 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.2-sgRNA7 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA8

Plasmid

#199734

Purpose

Golden Gate entry vector; 8th gRNA under TaU6 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU6-sgRNA8 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#176871

Purpose

MoClo Level 0 plasmid, contains AtUbi1 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJRA103

Plasmid

#209336

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_2

Plasmid

#91160

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)

Plasmid

#75412

Purpose

Module for the expression of the Renilla Luciferase with the silencing suppressor P19

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

Renilla / P19

Use

Luciferase and Synthetic Biology

Expression

Plant

Promoter

35S

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB602-mCherry-sfGFP

Plasmid

#213860

Purpose

For Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

mCherry-sfGFP

Expression

Bacterial and Plant

Promoter

CaMV 35S

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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MIGS4.1 (FF571)

Plasmid

#35251

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Expression

Plant

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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p33Fn

Plasmid

#158155

Purpose

Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Mutation

Rice codon optimized

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJG363

Plasmid

#91183

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_D10A+gNt_R2+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJG367

Plasmid

#91185

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_H840A+gNt_R2+gNt_F2+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJG370

Plasmid

#91187

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_D10A+gRNActrl+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJG382

Plasmid

#91189

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_D10A+gNt_R2+gNt_F2+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB135

Plasmid

#68589

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pGG-NES-TurboID

Plasmid

#209393

Purpose

Transiently expressing TurboID fused with a nuclear export signal (NES) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

NES-3XHA-TurboID

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-TurboID-GFP-PTS1

Plasmid

#209406

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID-PTS1

Tags

GFP

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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JAB2098

Plasmid

#190340

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt

Expression

Plant

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pORFE0011

Plasmid

#112075

Purpose

pcUBI promoter module Pro+5U

Depositor

Christian Chevalier

Insert

pcUBI promoter

Expression

Plant

Mutation

BsaI and BpiI restriction sites have been remove…

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFH56

Plasmid

#125882

Purpose

Level0 Rice OsACT1 Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsAct1 Pol II promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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mTALEAD 16S rRNA-1139T10C

Plasmid

#210838

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast

Expression

Plant

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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