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3TERM_Nos

Plasmid

#136115

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_Nos (A. tumefaciens)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pICSL1P6_crU626

Plasmid

#191781

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_crU626

Plasmid

#191778

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P4_crU626

Plasmid

#191779

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pZG71

Plasmid

#202577

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMAP L1Γ

Plasmid

#153345

Purpose

Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMAP L1B

Plasmid

#153344

Purpose

Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ239-RR

Plasmid

#108862

Purpose

FnCpf1 Gateway entry plasmid with N623R and K687R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RR (FnCpf1 with N623R and K687R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R and K687R, Cpf1 is rice codon optimized

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

Plasmid

#192412

Purpose

To act as a 1-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

Plasmid

#192413

Purpose

To act as a 1-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM843

Plasmid

#84169

Purpose

target miRNA843 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM843

Expression

Plant

Promoter

2x35S

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM845

Plasmid

#84170

Purpose

target miRNA845 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM845

Expression

Plant

Promoter

2x35S

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBI101.1/ProGOXL1:GUS

Plasmid

#175557

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL1

Tags

GUS

Expression

Plant

Promoter

GOXL1

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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lvl0 HSPt

Plasmid

#153378

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

HSP terminator

Expression

Plant

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25I

Plasmid

#91146

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSi

Plasmid

#67884

Purpose

Derivative of pSiM24 plant expression vector without the M24 promoter

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pSB115 - pL2_pSB90_fLUC-I_tGFP-I

Plasmid

#123190

Purpose

binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ269A2

Plasmid

#164714

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N

Plasmid

#189649

Purpose

rbc L 294 Left_G1397 N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Expression

Plant

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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