1,737 results

1. T444T-GFP(NCO)

   Plasmid

   #183972

   Purpose

   Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP

   Depositor

   David Matus

   Article

   Palmisano et al CourseSource, 2022

   Insert

   GFP (non-codon-optimized)
   

   Use

   RNAi

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF11-wemiRFP670-T2A-HO1

   Plasmid

   #197243

   Purpose

   Expresses the protein of wemiRFP670-T2A-HO1 in neurons of C elegan

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wemiRFP670-T2A-HO1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJW2172

   Plasmid

   #163096

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD364

   Plasmid

   #91831

   Purpose

   Lox511I-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Lox511I-SEC-Lox511I
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD382

   Plasmid

   #91830

   Purpose

   Lox2272-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Lox2272-SEC-Lox2272
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCC324

   Plasmid

   #110165

   Purpose

   miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   R61K change made in ERK-KTR CDS

   Promoter

   lin-31 promoter/enhancer

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGLOW39

   Plasmid

   #173068

   Purpose

   mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   mScarlet-I-C1^SEC^3xMyc
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xMyc and C. elegans codon-optimized mScarlet

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW2086

   Plasmid

   #154335

   Purpose

   Crispr repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::GFP::AID*::3xFLAG::10aa linker
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr

   Plasmid

   #19325

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Punc-122::GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHW533 (Peft-3::cGAL-N::let-858 3'UTR)

    Plasmid

    #107132

    Purpose

    cGAL-N driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans eft-3 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHW531 (Peft-3::cGAL-C::let-858 3'UTR)

    Plasmid

    #107136

    Purpose

    cGAL-C driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::gp41-1-C-intein::cGAL(AD)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans eft-3 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJL44 - Phsp16.48::MosTase::glh-2utr

    Plasmid

    #19333

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Phsp-16::Mos1 transposase
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBN267

    Plasmid

    #86723

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Prgef-1::FLP D5
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW1219

    Plasmid

    #61250

    Purpose

    CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHZ081

    Plasmid

    #27506

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pcol-10::lin-4::let-858_3'UTR (lin-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

    Plasmid

    #193852

    Purpose

    Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    Guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Prab-3::NLS::GFP

    Plasmid

    #68120

    Purpose

    Nuclear Localized GFP for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJH3449

    Plasmid

    #179576

    Purpose

    Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    BFP

    Expression

    Worm

    Mutation

    Promoter

    Plgc-55B

    Available since

    March 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGLOW77

    Plasmid

    #177338

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-2::mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    myo-2

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHW530 (Prab-3::cGAL-N::let-858 3'UTR)

    Plasmid

    #107131

    Purpose

    cGAL-N driver under the control of C. elegans rab-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans rab-3 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge