1,755 results

1. pCFJ150-Cas9(dpiRNA)

   Plasmid

   #107940

   Purpose

   eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   Cas9(dpiRNA)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   eft-3

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TC512

   Plasmid

   #122615

   Purpose

   Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   Zip(-)::QFAD::NLS::SL2::mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJW1584

   Plasmid

   #121055

   Purpose

   YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   YPET^SEC^AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, C. elegans codon optimized YPET, and auxi…

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMZ9

   Plasmid

   #198786

   Purpose

   Drives specific expression in the AWC (ON) neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   Psrt-28-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCC14

   Plasmid

   #198781

   Purpose

   Drives specific expression in the ASJ neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   Ptrx-1-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMZ10

   Plasmid

   #198787

   Purpose

   Drives specific expression in the AWC (OFF) neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   Psrt-47-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWP005

   Plasmid

   #198785

   Purpose

   Drives specific expression in the AWB neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   Pstr-1-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJL065

   Plasmid

   #198784

   Purpose

   Drives specific expression in the AWA neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   Podr-10-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWP061

   Plasmid

   #198775

   Purpose

   Drives specific expression in the ASE neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   Pgcy-5-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPuroG1

    Plasmid

    #200224

    Purpose

    puroR nls-mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHygRR2r

    Plasmid

    #200076

    Purpose

    hygR selection mScarlet 2X marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHygO2r

    Plasmid

    #200079

    Purpose

    hygR selection unmarked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159854

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMB66

    Plasmid

    #47946

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.48

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]

    Plasmid

    #159900

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mcherry | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pmlc-1

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUA97

    Plasmid

    #69340

    Purpose

    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

    Depositor

    Oliver Hobert

    Insert

    ceBFP SEC 3xFlag
    

    Use

    Tags

    3xFlag and ceBFP (mTagBFP)

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEY48

    Plasmid

    #191052

    Purpose

    klp-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    klp-6 (klp-6 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEY47

    Plasmid

    #191051

    Purpose

    gcy-21 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    gcy-21 (gcy-21 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJH3628

    Plasmid

    #191445

    Purpose

    Prgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural expression of Chrimson RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Chrimson
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCZGY2851

    Plasmid

    #87761

    Purpose

    Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Punc-17b-PH-miniSOG(Q103L)
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Promoter

    Punc-17b

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge