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TC476

Plasmid

#122629

Purpose

Punc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Publication

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Expression

Worm

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::GCaMP6s

Plasmid

#124351

Purpose

Nuclear Localized Pan-Neuronal GCaMP6s

Depositor

Andrew Leifer

Publication

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

rab-3, GCaMP6s (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGM48DEST

Plasmid

#74757

Purpose

Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Publication

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

QS
Gateway cassette

Expression

Worm

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSN444

Plasmid

#108331

Purpose

Expresses Sat2 for Nourseothricin resistance selection of C. elegans

Depositor

Shinsuke Niwa

Publication

Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

Insert

Streptothricin acetyl transferase 2

Expression

Worm

Promoter

rps-27 promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSF143-wArchon2-KGC-EGFP-ER2

Plasmid

#108430

Purpose

Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon2-KGC-EGFP-ER2

Expression

Worm

Promoter

flp-18

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHW531 (Peft-3::cGAL-C::let-858 3'UTR)

Plasmid

#107136

Purpose

cGAL-C driver under the control of C. elegans eft-3 promoter

Depositor

Paul Sternberg

Publication

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans eft-3 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHW533 (Peft-3::cGAL-N::let-858 3'UTR)

Plasmid

#107132

Purpose

cGAL-N driver under the control of C. elegans eft-3 promoter

Depositor

Paul Sternberg

Publication

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans eft-3 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDD399

Plasmid

#91821

Purpose

AID CT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2842

Plasmid

#87762

Purpose

Punc-25-PH::miniSOG, expression of membrane targeted PH-miniSOG in GABAergic motor neurons.

Depositor

Andrew Chisholm

Publication

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-25-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Punc-25

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBN260

Plasmid

#86722

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmyo-3::FLP D5

Expression

Worm

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBN278

Plasmid

#86724

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmec-7::FLP D5

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+ cMyc-oDam_f_GFPoNLS

Plasmid

#85818

Purpose

iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Publication

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

cMyc oDam_f_GFPoNLS

Tags

cMyc and oNLS (optimized nuclear localization sig…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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rgef-1p::NmBirA

Plasmid

#79971

Purpose

encodes E.coli biotin-ligase under neuronal promotor for C.elegans

Depositor

Baris Tursun

Publication

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Tags

NLS::myc

Expression

Worm

Promoter

rgef

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMB41

Plasmid

#73087

Purpose

N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering

Depositor

Mike Boxem

Publication

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

Avi-GFP tag

Tags

Avi and GFP

Expression

Worm

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pJW6.01

Plasmid

#62882

Purpose

Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

Depositor

Geraldine Seydoux

Publication

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GFP::MEG-3

Tags

GFP

Expression

Worm

Promoter

meg-3

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pKS2236

Plasmid

#37833

Depositor

Gary Silverman

Publication

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

YFP

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKS2311

Plasmid

#37835

Depositor

Gary Silverman

Publication

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2cpl-1W32A;Y35A::YFP (cpl-1 Nematode)

Tags

YFP

Expression

Worm

Mutation

W32A;Y35A

Promoter

nhx-2

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pAV1997

Plasmid

#37831

Depositor

Gary Silverman

Publication

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

mCherry

Expression

Worm

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFM032

Plasmid

#40076

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Type

Empty backbone

Tags

Dendra2 PAFP, S-peptide, and TEV protease cleavag…

Expression

Worm

Promoter

pie-1

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pKR2.40

Plasmid

#26952

Depositor

Geraldine Seydoux

Publication

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

pie-1promoter-GFP- gateway destination cassette B -pie-1 3’

Tags

GFP

Expression

Worm

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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