1,779 results

1. pCCM935

   Plasmid

   #58202

   Purpose

   Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

   Depositor

   Craig Mello

   Article

   Kim et al Genetics. 2014 May 30. pii: genetics.114

   Insert

   unc-22 sgRNA
   

   Expression

   Worm

   Promoter

   U6

   Available Since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

   Plasmid

   #182358

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   GFP
   

   Expression

   Worm

   Promoter

   Pmex-5

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ1663

   Plasmid

   #51484

   Purpose

   miniMos transposable element with HygroR and peel-1, MCS cloning

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ150-set2

   Plasmid

   #82546

   Purpose

   Pmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjection

   Depositor

   Sean Ryder

   Article

   Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

   Insert

   Pmex-5:GFP::H2B::PEST::set-2 3'UTR
   

   Expression

   Worm

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

   Plasmid

   #130275

   Purpose

   Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

   Insert

   Arch(D95N)
   

   Tags

   2x Myc-tag

   Expression

   Worm

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS716

   Plasmid

   #188888

   Purpose

   Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   floxed Cbr-unc-119 marker and Phsp16.41::Cre
   

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD288

   Plasmid

   #137017

   Purpose

   mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   mNG-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized mNG

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMLS262

   Plasmid

   #73718

   Purpose

   C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Psnt-1::2xNLS-FLP-D5
   

   Expression

   Worm

   Mutation

   G5D

   Promoter

   Psnt-1

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK73.ChrII_ttTi5605.sgRNA

   Plasmid

   #194058

   Purpose

   sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ChrII_ttTi5605 sgRNA
   

   Expression

   Worm

   Promoter

   U6

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTK73.ChrV_oxTi365.gRNA

    Plasmid

    #194052

    Purpose

    sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ChrV_oxTi365 sgRNA
    

    Expression

    Worm

    Promoter

    U6

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSDH61

    Plasmid

    #139033

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    epdz (no STOP codon)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJA327

    Plasmid

    #74486

    Purpose

    superfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    superfolder GFP
    

    Expression

    Worm

    Promoter

    myo-3

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

    Plasmid

    #159811

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ1655

    Plasmid

    #51481

    Purpose

    miniMos transposable element with HygroR, R4-R3 Gateway cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSM03

    Plasmid

    #139043

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    dendra2 (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ150-Cas9(dpiRNA)

    Plasmid

    #107940

    Purpose

    eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    Cas9(dpiRNA)
    

    Expression

    Worm

    Promoter

    eft-3

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSO221 Phis-72 BirA GFP

    Plasmid

    #45100

    Depositor

    Steven Henikoff

    Article

    Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

    Inserts

    BirA::GFP
    unc-119 (unc-119 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    his-72 and unc-119

    Available Since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. BSP188

    Plasmid

    #110917

    Purpose

    For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    unc54 Terminator
    

    Use

    Synthetic Biology; Yeast homologous recombination…

    Expression

    Worm

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMB66

    Plasmid

    #47946

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Promoter

    hsp-16.48

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSM_ser-2prom3p::myr-mCherry

    Plasmid

    #164223

    Purpose

    Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

    Depositor

    Yan Zou

    Article

    Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

    Insert

    myr-mCherry
    

    Expression

    Worm

    Promoter

    ser-2promo3

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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