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pPD95_81

Plasmid

#1497

Depositor

Andrew Fire

Type

Empty backbone

Tags

rf8134, IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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L4796

Plasmid

#1680

Depositor

Andrew Fire

Type

Empty backbone

Tags

let858enh>>, let858enh<<, let858-455,…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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L2603

Plasmid

#1511

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, ATG-SecSig, pes10

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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L3135

Plasmid

#1531

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, ATG-SecSig, pes10

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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L3522

Plasmid

#1547

Depositor

Andrew Fire

Type

Empty backbone

Tags

mec7-836, mec7Tsa, IVSA, gfpN, IVSR, gfpF64L, gfp…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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L3808

Plasmid

#1598

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, T7p, gfpN, IVSR, gfpS65C, IVS…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pAttBF3Hyg

Plasmid

#244885

Purpose

Hyg resistant attB phiC31 recombination-mediation insertion (RMI) vector for C. elegans

Depositor

Michael Nonet

Article

Beck et al MicroPubl Biol. 2025 Sep 2;2025:10.17912

Type

Empty backbone

Use

Phic31 recombinase mediated insertion vector

Expression

Worm

Promoter

none

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMS114

Plasmid

#215677

Purpose

Empty repair plasmid for ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxN + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMS59

Plasmid

#215680

Purpose

Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

Plasmid

#193048

Purpose

Heatshock expressed Cas9

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR

Use

CRISPR

Expression

Worm

Promoter

hsp16.41p

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

Plasmid

#193049

Purpose

Encodes G-418 resistance

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

prsp-27::NeoR::unc-54 3’ UTR

Expression

Worm

Promoter

prsp-27

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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PGK-EGFP-FKBPF36V

Plasmid

#199765

Purpose

plasmid for targeting dTAG into the AAVS1 locus

Depositor

Hannes Lans

Article

van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

Insert

GFP-dTAG

Tags

GFP and dTAG

Expression

Mammalian and Worm

Promoter

hPGK

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJR022

Plasmid

#198820

Purpose

C. elegans optimized version of mScarlet

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::wrmScarlet::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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AID-mTagBFP2-loxP_myo2_neoR-loxP

Plasmid

#194055

Purpose

Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

IAA17 (AXR3 Mustard Weed)

Tags

codon optimized mTagBFP2

Expression

Worm

Promoter

None

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Ttx-3-mCherry-SL2-Post-SPHERE-SF-iGluSnFR

Plasmid

#187100

Purpose

Glutamate sensor for localization to membrane contact

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-SF-iGluSnFR

Tags

mCherry-SL2

Expression

Worm

Promoter

Ttx-3

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJRK248

Plasmid

#173755

Purpose

co-injection plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR

Expression

Worm

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK261

Plasmid

#173754

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

(empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR

Expression

Worm

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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TC374

Plasmid

#164886

Purpose

Prpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rpl28-rtTA(Q)

Expression

Worm

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSM_ser-2prom3p::myr-mCherry

Plasmid

#164223

Purpose

Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

Depositor

Yan Zou

Article

Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

Insert

myr-mCherry

Expression

Worm

Promoter

ser-2promo3

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159811

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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