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pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

Plasmid

#182348

Purpose

mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJRK248

Plasmid

#173755

Purpose

co-injection plasmid

Depositor

Mike Boxem

Publication

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR

Expression

Worm

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJRK261

Plasmid

#173754

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Publication

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

(empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR

Expression

Worm

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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TC374

Plasmid

#164886

Purpose

Prpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,

Depositor

Tian Chi

Publication

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rpl28-rtTA(Q)

Expression

Worm

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSM_ser-2prom3p::myr-mCherry

Plasmid

#164223

Purpose

Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

Depositor

Yan Zou

Publication

Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

Insert

myr-mCherry

Expression

Worm

Promoter

ser-2promo3

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159811

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]

Plasmid

#159796

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Prpl-3 | histamine | rpl-3 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159862

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

Plasmid

#159896

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR

Expression

Worm

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4531

Plasmid

#132366

Purpose

Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Tags

none

Expression

Worm

Promoter

Prgef-1

Available since

Sept. 22, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pJW1839

Plasmid

#154325

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3725

Plasmid

#154113

Purpose

UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

UAS 11X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSM08

Plasmid

#139042

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mscarlet (includes STOP codon)

Expression

Worm

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCC336

Plasmid

#110167

Purpose

miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

Depositor

Iva Greenwald

Publication

de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

Insert

ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B

Use

Minimos transposon

Tags

mCherry and mClover

Expression

Worm

Mutation

Phospho-acceptor sites mutated: S43E, T55E, T62E.…

Promoter

lin-31 promoter/enhancer

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCC335

Plasmid

#110166

Purpose

miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

Depositor

Iva Greenwald

Publication

de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

Insert

ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B

Use

Minimos transposon

Tags

mCherry and mClover

Expression

Worm

Mutation

Phospho-acceptor sites mutated: S43A, T55A, T62A.…

Promoter

lin-31 promoter/enhancer

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

Plasmid

#130275

Purpose

Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Publication

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

Arch(D95N)

Tags

2x Myc-tag

Expression

Worm

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pNH12 (pmyo-2::MacQ::mCitrine)

Plasmid

#130274

Purpose

Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Publication

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

MacQ::mCitrine

Tags

mCitrine

Expression

Worm

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his24::tagBFP2

Plasmid

#124344

Purpose

Nuclear Localized Pan-Neuronal tagBFP2

Depositor

Andrew Leifer

Insert

rab3, tagBFP2 (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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BSP608

Plasmid

#122253

Purpose

expression vector for mNeptune under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mNeptune

Expression

Worm

Promoter

EFT3

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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