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pCZ829

Plasmid

#110137

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Publication

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito-GCaMP5

Tags

mito::GCaMP5

Expression

Worm

Promoter

Pcol-19

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)

Plasmid

#107135

Purpose

cGAL-C driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Publication

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans myo-2 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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(p)odr-3::GFP::EGL-4

Plasmid

#100897

Purpose

GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

Depositor

Noelle L'Etoile

Publication

Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

Insert

56-2741: odr-3 promoter

Expression

Worm

Promoter

odr-3

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pBN312

Plasmid

#86734

Purpose

GFP with Frt sites for FLP-mediated knock-out of gene function in C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

GFP_Frt_KO

Expression

Worm

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBN67

Plasmid

#86714

Purpose

DamID control plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

Insert

Phsp16.41::GFP::MYCDam

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBN282

Plasmid

#86727

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pelt-2::FLP D5

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBN283

Plasmid

#86728

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pdat-1::FLP D5

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+ oDam_f_GFPoNLS

Plasmid

#85819

Purpose

iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Publication

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

oDam_f_GFPoNLS

Tags

oNLS (optimized nuclear localization signal) C te…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Pmyo3::tag:sl1(G16C)

Plasmid

#79003

Purpose

Expression of exogenous SL1 in C. elegans muscle

Depositor

Xiao Liu, Ge Zhan

Publication

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Tags

illumina-adapter

Expression

Worm

Mutation

G16C

Promoter

myo-3

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS286

Plasmid

#73723

Purpose

Saptrap tagRFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

tagRFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS291

Plasmid

#73724

Purpose

SapTrap mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AP568-3

Plasmid

#70048

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Publication

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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AP568-2

Plasmid

#70047

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Publication

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJM656

Plasmid

#53086

Purpose

A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control

Depositor

Ding Xue

Publication

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Lactaherin (Mfge8 Mouse)

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Mutation

deleted native signal sequence, deleted C-termina…

Promoter

hsp-16.41

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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sgRNA with rpr-1 promoter

Plasmid

#48961

Purpose

to drive the sgRNA expression under RNase P non-coding RNA promoter

Depositor

Mario de Bono

Publication

Chen et al Nucleic Acids Res. 2013 Sep 5.

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

rpr-1 promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRG02

Plasmid

#44196

Depositor

Denis Dupuy

Insert

Neo

Expression

Worm

Promoter

rps-27

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKS2301

Plasmid

#37834

Depositor

Gary Silverman

Publication

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2cpl-1::YFP (cpl-1 Nematode)

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pPK719

Plasmid

#38149

Depositor

Patricia Kuwabara

Insert

cbr-unc-119::FRT*::galk::FRT* (Cbr-unc-119 Nematode)

Use

Recombineering

Expression

Worm

Promoter

unc-119

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPD95.77-wVenus

Plasmid

#37465

Depositor

Hitoshi Sawa

Publication

Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

Type

Empty backbone

Expression

Worm

Promoter

none

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJM601-TRA-2::FLAG

Plasmid

#27129

Depositor

Ding Xue

Publication

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 FLAG (tra-2 Nematode)

Tags

3x FLAG

Expression

Worm

Mutation

Replaced TRA-2 stop codon with KpnI and EcoRI and…

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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