1,779 results

1. pSR40

   Plasmid

   #69257

   Purpose

   plin-31::CRE for integration on ttTi14024, Chr X

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox; Mossci

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   lin-31

   Available Since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJH4.52

   Plasmid

   #26946

   Depositor

   Geraldine Seydoux

   Article

   Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

   Insert

   histone H2B (his-58 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Available Since

   June 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

   Plasmid

   #182350

   Purpose

   Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBCN22-R4R3

   Plasmid

   #26302

   Depositor

   Ben Lehner

   Article

   Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST- IP2.0(codon for C.elegans)

   Plasmid

   #109216

   Purpose

   IP2.0 for C. elegans (Codon optimization and introns).

   Depositor

   Takeshi Ishihara

   Article

   Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

   Insert

   IP2.0 (Inverse Pericam 2.0)
   

   Tags

   His-tag

   Expression

   Worm

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSR02

   Plasmid

   #69149

   Purpose

   Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Inserts

   mCherry
   eGFP
   

   Use

   Cre/Lox; Mossci

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   rps-27

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-YFP-Smo

   Plasmid

   #31618

   Depositor

   Rajat Rohatgi

   Article

   Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

   Insert

   Smo (Smo Mouse)
   

   Use

   Zebrafish, avian expression

   Tags

   YFP

   Expression

   Mammalian and Worm

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pEY12

   Plasmid

   #191018

   Purpose

   Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   rab-3 (rab-3 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMLS286

   Plasmid

   #73723

   Purpose

   Saptrap tagRFP + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   tagRFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEY46

    Plasmid

    #191050

    Purpose

    glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    glr-1 (glr-1 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEY38

    Plasmid

    #191042

    Purpose

    unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    unc-8 (unc-8 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEY13

    Plasmid

    #191019

    Purpose

    Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    arrd-4 (ZK938.2 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEY20

    Plasmid

    #191026

    Purpose

    gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    gcy-18 (gcy-18 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid

    #108362

    Purpose

    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

    Depositor

    Luisa Cochella

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Inserts

    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    

    Tags

    2xFlag, MYC, T2A peptide, and TY1 linker

    Expression

    Worm

    Promoter

    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

    Plasmid

    #129538

    Purpose

    modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

    Depositor

    Arneet Saltzman

    Article

    Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox; Mos1-mediated single copy insertion (mos…

    Expression

    Worm

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGM48DEST

    Plasmid

    #74757

    Purpose

    Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

    Depositor

    Jordan Ward, Keith Yamamoto

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Inserts

    QS
    Gateway cassette
    

    Expression

    Worm

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

    Plasmid

    #168170

    Purpose

    Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
    

    Tags

    YFP

    Expression

    Worm

    Mutation

    E497K and C566D

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJR025

    Plasmid

    #198832

    Purpose

    Actin filament and cell cortex localization.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::act2-venus::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMB62

    Plasmid

    #47944

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAS1-rab-3P::his-24::CyOFP

    Plasmid

    #124347

    Purpose

    Nuclear Localized Pan-Neuronal CyOFP

    Depositor

    Andrew Leifer

    Insert

    rab-3, CyOFP (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge