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pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

Plasmid

#129538

Purpose

modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

Depositor

Arneet Saltzman

Article

Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

Type

Empty backbone

Use

Cre/Lox; Mos1-mediated single copy insertion (mos…

Expression

Worm

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168170

Purpose

Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Mutation

E497K and C566D

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD133.47

Plasmid

#27510

Depositor

Andrew Fire

Article

Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

Insert

pmyo-3::cfp::lacZ::unc-54_3'UTR

Expression

Worm

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pIN4_tsp-6_mScarlet

Plasmid

#182935

Purpose

Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Promoter

tsp-6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJH2523

Plasmid

#191358

Purpose

Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GCaMP3

Tags

mCherry

Expression

Worm

Promoter

Punc-25

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJR025

Plasmid

#198832

Purpose

Actin filament and cell cortex localization.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::act2-venus::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJH4.67

Plasmid

#26948

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

gamma-tubulin (tbg-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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BSP606

Plasmid

#122251

Purpose

expression vector for mECitrine under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mECitrine

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJH4733

Plasmid

#178898

Purpose

Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pmyo-3

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMB60

Plasmid

#47941

Purpose

Can be used to generate synthetic guide in vitro from T7 promotor

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

synthetic guide RNA

Use

CRISPR

Expression

Worm

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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TU#707

Plasmid

#15276

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzgfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pKPY197

Plasmid

#62599

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr412Gly-CePheRS Alpha Subunit

Tags

HIS

Expression

Worm

Mutation

Thr412Gly

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pJRK249

Plasmid

#173747

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

Plasmid

#182359

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Ultra-pan-neuronal positive control pUNP::gfp(NLS)

Expression

Worm

Promoter

Promoter Ultra Pan Neuronal

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY2845

Plasmid

#87763

Purpose

Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pnmr-1-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pnmr-1

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pPD176.36

Plasmid

#1720

Depositor

Andrew Fire

Type

Empty backbone

Tags

T7p, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, lacIN…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pJH2217

Plasmid

#179251

Purpose

Psra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Mutation

M280I

Promoter

Psra-11

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDO001

Plasmid

#198828

Purpose

Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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